DIA-based phosphoproteomics profiling reveals rapid cold-responsive phosphorylation events in tomato fruit

文献类型： 外文期刊

第一作者： Tan, Jinjuan

作者： Tan, Jinjuan;Feng, Hanqian;Deng, Zhiping

作者机构：

关键词： Solanum lycopersicum; Proteomics; Phosphosites; Chilling injury; Postharvest

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：6.8； 五年影响因子：7.5 ）

ISSN： 0925-5214

年卷期： 2025 年 219 卷

页码：

收录情况： SCI

摘要： Low-temperature storage is widely used to extend the shelf life of fresh fruit and vegetables, but it often induces chilling injury, leading to quality loss and consumer dissatisfaction. While numerous studies have explored the molecular mechanisms of cold response in fruit, primarily focusing on long-term cold treatments, the early signaling events of cold response in harvested fruit remain largely unknown. In this study, we constructed mass spectral libraries of the proteome (over 11,000 proteins) and phosphoproteome (over 24,000 high-confidence phosphosites) from tomato fruit. We performed data-independent acquisition (DIA)-based comprehensive proteome and phosphoproteome profiling of tomato fruit at the red-ripe stage following a 30-minute cold treatment. Approximately ten percent of the quantified phosphopeptides were significantly altered, with no observed changes in protein abundance. Functional enrichment analysis of cold-regulated phosphoproteins suggests involvement in diverse cold-responsive biological processes and pathways, distinct from those reported in longterm cold-stress phosphoproteomes, including plant-pathogen interaction and the MAPK signaling pathway. We identified many regulators of fruit development and ripening that respond to cold shock at the protein phosphorylation level, such as ethylene-associated ripening regulators (EIN2, TPR1, FERL, E8, REM1), phytohormone-related proteins (BZR1/BES1, PIN4, ARF8a, LoxB), the flavor volatile-controlling lipoxygenase LoxC, and key transcription factors (CNR, WRKY35). This suggests extensive crosstalk between ripening and cold signal transduction. Additionally, we identified 109 kinases, 40 E3 ubiquitin ligases, and 39 transcription factors as cold-stimulus responsive phosphoproteins. Our study constructs the most extensive mass spectral libraries of tomato fruit (phospho)proteins to date and provides a detailed landscape of rapid and extensive cold-responsive phosphorylation events in tomato fruit.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序