The m6A reader SlYTH2 negatively regulates tomato fruit aroma by impeding the translation process

文献类型： 外文期刊

第一作者： Bian, Hanxiao

作者： Bian, Hanxiao;Gao, Ying;Wang, Hanqing;Ye, Bingbing;Chen, Kunsong;Klee, Harry J.;Zhang, Bo;Song, Peizhe;Cai, Zhihe;Jia, Guifang;Gao, Ying;Deng, Zhiping;Huang, Chenyang;Liu, Jianzhao;Yu, Lei;Gao, Xiangwei;Pan, Yu;Wang, Fengqin;Jia, Guifang;Jia, Guifang;Zhang, Bo

作者机构：

关键词： phase separation; translation repression; RNA methylation; flavor quality

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：9.4； 五年影响因子：10.8 ）

ISSN： 0027-8424

年卷期： 2024 年 121 卷 28 期

页码：

收录情况： SCI

摘要： N 6- methyladenosine (m 6 A) is a fundamentally important RNA modification for gene regulation, whose function is achieved through m 6 A readers. However, whether and how m 6 A readers play regulatory roles during fruit ripening and quality formation remains unclear. Here, we characterized SlYTH2 as a tomato m 6 A reader protein and profiled the binding sites of SlYTH2 at the transcriptome- wide level. SlYTH2 undergoes liquid- liquid phase separation and promotes RNA-protein condensate formation. The target mRNAs of SlYTH2, namely m 6 A- modified SlHPL and SlCCD1B associated with volatile synthesis, are enriched in SlYTH2- induced condensates. Through polysome profiling assays and proteomic analysis, we demonstrate that knockout of SlYTH2 expedites the translation process of SlHPL and SlCCD1B , resulting in augmented production of aroma- associated volatiles. This aroma enrichment significantly increased consumer preferences for CRISPR- edited fruit over wild type. These findings shed light on the underlying mechanisms of m 6 A in plant RNA metabolism and provided a promising strategy to generate fruits that are more attractive to consumers.

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