Fluorescence determination of 3-MCPD by combining amino silica nanoparticles with fluorescein isothiocyanate
文献类型： 外文期刊
第一作者： Xu, Ting
作者： Xu, Ting;Qingru, Zeng;Fu, Qing;Wang, Zhaojie;Liu, Xin;Xiao, Shensheng;Gong, Zhiyong;Fang, Min;Liu, Xin;Xiao, Shensheng;Gong, Zhiyong;Fang, Min;Jiang, Xiaoming;Lu, Yuepeng;Wu, Yongning
作者机构：
关键词： 3-MCPD; FITC; Amino silica; Fluorescence analysis; Rapid detection
期刊名称：JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY （ 影响因子：2.4； 五年影响因子：3.4 ）
ISSN： 2093-3134
年卷期： 2023 年 14 卷 1 期
页码：
收录情况： SCI
摘要： Using amino silica modified with fluorescein isothiocyanate (FITC), a quick fluorescence analysis technique is used for the detection of 3-monochloropropane-1,2-diol (3-MCPD). At 100 degrees C and pH 8.5, FITC-modified amino silica exhibits the lowest fluorescence intensity in the presence of 3-MCPD. This can predominantly be explained mostly explained by 3-MCPD's capacity to occupy the amino group that FITC normally binds to. The fluorescence intensity of FITC-modified amino silica was greatly quenched by 3-MCPD's reaction with the amino group under alkaline conditions, and the fluorescence intensity is different at different reaction times, reaction pH, and reaction temperature. The effects of various 3-MCPD concentrations on the optical characteristics of FITC-modified amino silica were also investigated. Fluorescence analysis is used to obtain a linear range from 0.025 to 1.0 mg/L for 3-MCPD detection under optimal experimental conditions, with a detection limit of 0.025 mg/L and a correlation coefficient of 0.9915. The quantity of 3-MCPD in soy sauce was measured under ideal conditions. Using the optimized conditions, the contents of 3-MCPD in soy sauce were determined. These results suggest that this method is sensitive to 3-MCPD and may have a substantial application in the rapid detection of food contaminants particularly, where the quality and safety of food products are of paramount concern.
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