Liquid hot water pretreatment combined with high-solids enzymatic hydrolysis and fed-batch fermentation for succinic acid sustainable processed from sugarcane bagasse

文献类型： 外文期刊

第一作者： Xu, Chao

作者： Xu, Chao;Xie, Chunliang;Gong, Wenbing;Zhu, Zuohua;Zhou, Yingjun;Peng, Yuande;Xiong, Yaru;Xu, Chao;Zhang, Jun;Li, Kuntai;Zhong, Saiyi;Huang, Shushi

作者机构： Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Peoples R China;Hunan Prov Ctr Dis Control & Prevent, Changsha 410005, Peoples R China;Changsha Univ Sci & Technol, Sch Food & Bioengn, Changsha 410005, Peoples R China;Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;Guangxi Key Lab Marine Nat Prod & Combinatorial Bi, Nanning 530007, Peoples R China

关键词： Liquid hot water; Sugarcane bagasse; Enzymatic hydrolysis; Succinic acid; Fermentation

期刊名称：BIORESOURCE TECHNOLOGY （ 2022影响因子：11.4； 五年影响因子：10.6 ）

ISSN： 0960-8524

年卷期： 2023 年 369 卷

页码：

收录情况： SCI

摘要： In order to sustainable process of bio-succinic acid (SA), response surface methodology (RSM) was applied to optimize liquid hot water pretreatment pretreatment of sugarcane bagasse (SCB), followed by high-solids enzymatic hydrolysis of pretreated residual that without washing, then the hydrolysates and partial pretreatment liquid were used as carbon sources for SA fermentation. Results showed that the highest sugars yield could be achieved at pretreatment conditions of temperature 186 degrees C, time 25 min and solid-to-liquid ratio 0.08; enzymatic digestion the pretreated residuals at 20 % (w/v) solid content via enzymes reconstruction and fedbatch strategy, the obtained sugars reached to 121 g/L; by controlling the nutrition and conditions of the fermentation process, most of the C5 and C6 sugars in the hydrolysate and pretreatment liquid were converted into SA with a conversion rate high to 280 mg/g SCB. This study can provide a novel clue for clean and efficient biorefining of chemicals.

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