Three in one: An effective and universal vaccine expressing heterologous tandem RBD trimer by rabies virus vector protects mice against SARS-CoV-2

文献类型： 外文期刊

第一作者： Huang, Jingbo

作者： Huang, Jingbo;Li, Hailun;Bai, Yujie;Song, Yumeng;Jiao, Cuicui;Huang, Pei;Zhang, Haili;Li, Yuanyuan;Wang, Hualei;Huang, Jingbo;Li, Hailun;Bai, Yujie;Song, Yumeng;Jiao, Cuicui;Huang, Pei;Zhang, Haili;Li, Yuanyuan;Wang, Hualei;Huang, Jingbo;Wang, Weiqi;Xia, Xianzhu;Yan, Feihu;Jin, Hongli

作者机构：

关键词： SARS-CoV-2; Tandem RBD-Heterotrimer; Immunogens; RBD; Recombinant rabies virus; Universal vaccine

期刊名称：ANTIVIRAL RESEARCH （ 影响因子：7.6； 五年影响因子：5.8 ）

ISSN： 0166-3542

年卷期： 2024 年 227 卷

页码：

收录情况： SCI

摘要： The rapid emergence of Severe Acute Respiratory Syndrome Coronavirus type 2 (SARS-CoV-2) variants, coupled with severe immune evasion and imprinting, has jeopardized the vaccine efficacy, necessitating urgent development of broad protective vaccines. Here, we propose a strategy employing recombinant rabies viruses (RABV) to create a universal SARS-CoV-2 vaccine expressing heterologous tandem receptor-binding domain (RBD) trimer from the SARS-CoV-2 Prototype, Delta, and Omicron strains (SRV-PDO). The results of mouse immunization indicated that SRV-PDO effectively induced cellular and humoral immune responses, and demonstrated higher immunogenicity and broader SARS-CoV-2 neutralization compared to the recombinant RABVs that only expressed RBD monomers. Moreover, SRV-PDO exhibited full protection against SARS-CoV-2 in the challenge assay. This study demonstrates that recombinant RABV expressing tandem RBD-heterotrimer as a multivalent immunogen could elicit a broad-spectrum immune response and potent protection against SARS-CoV-2, making it a promising candidate for future human or veterinary vaccines and offering a novel perspective in other vaccine design.

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