A universal automated method for determining the bacteriostatic activity of nanomaterials

文献类型： 外文期刊

第一作者： Zhang, Xuzhi

作者： Zhang, Xuzhi;Wang, Xiaochun;Zhao, Jun;Qu, Keming;Zhang, Xuzhi;Qu, Keming;Cheng, Hongrui;Zheng, Yuanhui

作者机构：

关键词： Multi-channel contactless conductometric sensor; Bacterial growth; Nanomaterials; Phenotypic antibiotic susceptibility testing; Universal method

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：10.588； 五年影响因子：10.129 ）

ISSN： 0304-3894

年卷期： 2021 年 413 卷

页码：

收录情况： SCI

摘要： The lack of analytical strategies to directly determine the bacteriostatic activity of nanomaterials in complex aqueous media (e.g., environmentally relevant scenarios) seriously hampers the harvest of reliable data for nanomaterial risk assessment. Here, we created an automated phenotypic method based on a developed multi-channel contactless conductometric sensor. Bacterial growth kinetics of E. coli and S. aureus were determined via on-line monitoring of conductivity changes in simple media (e.g., liquid LB broth) and complex media (e.g., relevant river water and seawater samples with diverse pH, salinity, conductivity, turbidity, chemical oxygen demand and total suspended solids). The high temporal resolution growth curves provide detailed information on the bacteria inhibition of the model nanomaterial - Au nanospheres, Au nanorods, Ag nanospheres and Ag nanocubes - at each growth stage, thus enabling users to directly obtain minimum inhibitory concentrations. The method highlights the advantages of universality, simplicity and affordability. It opens up possibilities for the development of a powerful analytical platform for researches in the field of nanoscience, e.g. to assess ecotoxicity of nanomaterials.

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