Transcriptomics and Metabolomics Combined to Analyze the Response Mechanism of Silkworm Eggs to High-Temperature Stress

文献类型： 外文期刊

第一作者： Xiao, Yang

作者： Xiao, Yang;Li, Qingrong;Sun, Zhenbo;Fu, Bing;Yang, Qiong;Zhang, Weilong;Xing, Dongxu;Jiang, Mangui;Huang, Xuhua

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关键词： transcriptomics; metabonomics; silkworm egg; instant acid-impregnated eggs; high temperature stress

期刊名称：INSECTS （ 影响因子：2.9； 五年影响因子：3.3 ）

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年卷期： 2025 年 16 卷 8 期

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收录情况： SCI

摘要： Conventional immediate and high-temperature immediate acid treatment are crucial techniques for breaking the diapause state of silkworm eggs, but their molecular mechanisms remain unclear. This study prepared diapause eggs (CK), conventional immediate acid-treated eggs (46 degrees C, 5 min, and CG), and high-temperature immediate acid-treated eggs (47.5 degrees C, 7 min, and GW) and analyzed the transcriptome and metabolome to screen for key expressed genes and key metabolites. Transcriptome results showed that 688, 823, and 222 differentially expressed genes (DEGs) were obtained from CK vs. CG, CK vs. GW, and CG vs. GW, respectively, and 12 DEGs significantly upregulated in all three comparisons (CK vs. CG, CK vs. GW, and CG vs. GW), including glycine-N-methyltransferase, choline dehydrogenase, Hsp68, and Hsp70. The LC-MS analysis results showed that 854, 711, and 506 differential metabolites (DMs) were obtained from CK vs. CG, CK vs. GW, and CG vs. GW, respectively. A total of seven DMs upregulated in all three comparisons and with |log2Fold Change| >= 0.5 in CG vs. GW, including tyrosine-isoleucine-histidine, phenylalanyl-tyrosine, tyrosine-phenylalanine-glutamate-lysine, and histidylleucine, as well as 12 downregulated DMs, were identified. Additionally, it was found that gamma-linolenic acid and triglycerides were upregulated in CG vs. GW. The conjoint analysis results revealed that four small peptides, including tyrosine-isoleucine-histidine, phenylalanyl-tyrosine, tyrosine-phenylalanine-glutamate-lysine, and histidylleucine, exhibited a highly significant positive correlation with Hsp70 family genes such as Hsp68 and Hsp70. This suggests that these small peptides, along with gamma-linolenic acid and triglycerides, may play a crucial role in the resistance of silkworm eggs to high-temperature stress and the associated oxidative stress.

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