Analysis of expression characteristics of soybean leaf and root tissue-specific promoters in Arabidopsis and soybean

文献类型： 外文期刊

第一作者： Xun, Hongwei

作者： Xun, Hongwei;Dong, Yingshan;Guo, Dongquan;Xun, Hongwei;Zhang, Xue;Yu, Jiamiao;Pang, Jinsong;Liu, Bao;Jiang, Lili;Wang, Shucai

作者机构：

关键词： Soybean; Tissue-specific promoter; GUS staining; Salt resistance; Arabidopsis thaliana

期刊名称：TRANSGENIC RESEARCH （ 影响因子：2.788； 五年影响因子：2.377 ）

ISSN： 0962-8819

年卷期： 2021 年 30 卷 6 期

页码：

收录情况： SCI

摘要： The characterization of tissue-specific promoters is critical for studying the functions of genes in a given tissue/organ. To study tissue-specific promoters in soybean, we screened tissue-specific expressed genes using published soybean RNA-Seq-based transcriptome data coupled with RT-PCR analysis. We cloned the promoters of three genes, GmADR1, GmBTP1, and GmGER1, and constructed their corresponding beta-Glucuronidase (GUS) promoter-GUS reporter vectors. We generated transgenic Arabidopsis plants and examined the expression patterns of these promoters by GUS staining and RT-PCR analysis. We also transformed the promoter-GUS reporter vectors into soybean to obtain hairy roots, and examined promoter expression by GUS staining. We found a root-specific expression pattern of GmADR1 and GmBTP1 in both Arabidopsis and soybean, and the promoter of GmGER1 showed a leaf-specific pattern in transgenic Arabidopsis plants. To test the potential utility of these promoters in soybean improvement by transgenic means, we used the GmADR1 promoter to drive expression of a salt resistance gene in soybean, GmCaM4, by generating transgenic soybean plants. We found that the transgenic plants had significantly enhanced salt tolerance compared to non-transformed wild-type, suggesting that introducing endogenous promoters by transgenic means can drive the expression of functional genes in specific tissues and organs in soybean.

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