Technology Invention and Mechanism Analysis of Rapid Rooting of Taxus x media Rehder Branches Induced by Agrobacterium rhizogenes

文献类型： 外文期刊

第一作者： Wang, Ying

作者： Wang, Ying;Luo, Xiumei;Su, Haotian;Guan, Ge;Liu, Shuang;Ren, Maozhi;Wang, Ying;Ren, Maozhi;Su, Haotian;Guan, Ge;Liu, Shuang;Ren, Maozhi

作者机构：

关键词： Taxus x media Rehder; Agrobacterium rhizogenes; branch rooting; paclitaxel

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 1 期

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收录情况： SCI

摘要： Taxus, a vital source of the anticancer drug paclitaxel, grapples with a pronounced supply-demand gap. Current efforts to alleviate the paclitaxel shortage involve expanding Taxus cultivation through cutting propagation. However, traditional cutting propagation of Taxus is difficult to root and time-consuming. Obtaining the roots with high paclitaxel content will cause tree death and resource destruction, which is not conducive to the development of the Taxus industry. To address this, establishing rapid and efficient stem rooting systems emerges as a key solution for Taxus propagation, facilitating direct and continuous root utilization. In this study, Agrobacterium rhizogenes were induced in the 1-3-year-old branches of Taxus x media Rehder, which has the highest paclitaxel content. The research delves into the rooting efficiency induced by different A. rhizogenes strains, with MSU440 and C58 exhibiting superior effects. Transcriptome and metabolome analyses revealed A. rhizogenes' impact on hormone signal transduction, amino acid metabolism, zeatin synthesis, and secondary metabolite synthesis pathways in roots. LC-MS-targeted quantitative detection showed no significant difference in paclitaxel and baccatin III content between naturally formed and induced roots. These findings underpin the theoretical framework for T. media rapid propagation, contributing to the sustainable advancement of the Taxus industry.

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