Guard cell and whole plant expression of ACTOR improves performance under drought and enhances water use efficiency

文献类型： 外文期刊

第一作者： Liu, Li

作者： Liu, Li;Gao, Peng;Sheng, Huajin;Bakshi, Achala;Schneider, David;Burbridge, Connor;Nguyen, Hanh;Wang, Sheng;Mora-Macias, Javier;Sharpe, Andrew;Datla, Raju;Kochian, Leon;Xiang, Daoquan;Babic, Vivijan;Ren, Maozhi;Armenta-Medina, Alma;Pozniak, Curtis;Magalhaes, Jurandir

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期刊名称：JOURNAL OF BIOLOGICAL CHEMISTRY （ 影响因子：3.9； 五年影响因子：4.3 ）

ISSN：

年卷期： 2025 年 301 卷 6 期

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收录情况： SCI

摘要： Water use efficiency is an important target for breeding of improved drought resistance. Minimizing leaf transpirational water loss plays a key role in drought resistance. But this reduces CO2 levels in leaves, which often reduces photosynthetic efficiency and yield. Signaling pathways play important roles in stress responses, and identifying the molecular, biochemical, and physiological determinants underlying drought signaling may offer new drought mitigating strategies. To explore these possibilities, and because of the importance of stomata in drought response and photosynthesis, we employed guard cell (GC)-targeted and constitutive overexpression of the Target of Rapamycin (TOR) kinase, a master regulator of signaling networks, in transgenic Arabidopsis. To investigate the impact of these ACTOR transgenes in drought, we conducted physiological and molecular investigations into drought responses, including leaf water loss, photosynthetic CO2 assimilation, stomatal H2O/CO2 conductance, leaf chlorophyll content, and global gene expression in response to drought in wild-type and ACTOR-expressing Arabidopsis. Links between both guard cell-localized and whole plant ACTOR overexpression were identified, revealing TOR is involved in conservation of water and sustained photosynthetic performance, along with identifica-tion of genes associated with drought response in WT versus ACTOR-expressing transgenic lines. These findings suggest that targeted guard cell ACTOR expression should help achieve a balance between plant water conservation during drought, and maintaining plant performance, by minimizing reductions in photosynthesis. Manipulation of guard cell ACTOR expression could be an effective avenue for developing crops with enhanced drought resistance and increased yield under drought stress, resulting in enhanced water use efficiency.

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