Secretory expression of porcine interferon-gamma in baculovirus using HBM signal peptide and its inhibition activity on the replication of porcine reproductive and respiratory syndrome virus

文献类型: 外文期刊

第一作者: Wang, Yan-Bin

作者: Wang, Yan-Bin;Wang, Zhen-Ya;Chen, Hong-Ying;Cui, Bao-An;Wang, Ya-Bin;Zhang, Hong-Ying;Wang, Rui

作者机构:

关键词: Porcine;Interferon-gamma;Baculovirus;HBM signal peptide;Inhibition activity;PRRSV

期刊名称:VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY ( 影响因子:2.046; 五年影响因子:2.217 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The gene sequence encoding mature porcine interferon-gamma (PoIFN- gamma ) fused with a C-terminal 6x histidine tag was cloned into the baculovirus pFastBac(TM) Dual vector of the Bac-to-Bac Baculovirus expression system under the control of PH promoter. The authentic signal sequence of porcine interferon-gamma was substituted with the honeybee melittin (HBM) signal sequence, and expressed in insect cells. The recombinant proteins were detected by SDS-PAGE and immunofluorescence assay. The nickel affinity column purified recombinant porcine interferon-gamma with HBM signal peptide (rPoIFN- gamma H) was shown to be a 19kDa protein as confirmed by Western blot analysis. The recombinant PoIFN- gamma H was shown to have cytokine activity, inhibiting the cytopathic effect of vesicular stomatitis virus (VSV) in PK-15 cells at about 1.07x10 super(6)U/mL. The 2 super(-) super(7) dilution of the rPoIFN- gamma H in culture supernatant protected the MARC-145 cells from the cytopathic effect caused by 100TCID sub(5) sub(0) of porcine reproductive and respiratory syndrome virus.

分类号: S85

  • 相关文献

[1]Antiviral effect of recombinant equine interferon-gamma on several equine viruses. Sentsui, Hiroshi,Wu, Donglai,Murakami, Kenji,Kondo, Takashi,Matsumura, Tomio. 2010

[2]The ecdysteroid UDP-glucosyltransferase gene promoter from Autographa californica multicapsid nucleopolyhedrovirus. Shen, XJ,Yi, YZ,Tang, SM,Zhang, ZF,Li, YR,He, JL.

[3]The genome sequence of the multinucleocapsid nucleopolyhedrovirus of the Chinese oak silkworm Antheraea pernyi. Fan, Qi,Li, Shuying,Wang, Linmei,Zhang, Bo,Ye, Bo,Zhao, Zhenjun,Cui, Liwang.

[4]Heterocycle-functional gramine analogues: Solvent- and catalyst-free synthesis and their inhibition activities against cell proliferation. Ke, Shaoyong,Shi, Liqiao,Yang, Qingyu,Liang, Ying,Yang, Ziwen,Cao, Xiufang.

[5]Course of infection by Babesia sp BQ1 (Lintan) and B-divergens in sheep depends on the production of IFN gamma and IL10. Moreau, E.,Guan, G.,Yin, H.,Luo, J.. 2010

[6]Effects of different doses of free alpha-linolenic acid infused to the duodenum on the immune function of lactating dairy cows. Sun, Peng,Wang, Jiaqi,Yang, Guang,Liu, Qingsheng.

[7]Regulation network of serum cytokines induced by tuberculosis-specific antigens reveals biomarkers for tuberculosis diagnosis. Wei, M.,Wu, Z. Y.,Lin, J. H.,Xie, Y. Q.,Su, H.,Zhou, W.,Li, Y.,Qian, Z. X.. 2015

[8]Cloning and expression of mink (Neovison vison) interferon-gamma gene and development of an antiviral assay. Zhang, Hailing,Zhao, Jianjun,Bai, Xue,Zhang, Lei,Hu, Bo,Liu, Hao,Xu, Shujuan,Yan, Xijun,Zhang, Hailing,Yan, Xijun,Zhang, Dongliang,Fan, Sining.

[9]Expression of biologically active recombinant equine interferon-gamma in Escherichia coli. Bai, Yu,Tong, Tiegang,Liu, Guangliang,Chen, Weiye,Zhang, Weijun,Wang, Qun,Yang, Tao,Bu, Zhigao,Wu, Donglai,Bai, Yu. 2010

[10]Spodoptera litura multicapsid nucleopolyhedrovirus inhibits Microplitis bicoloratus polydnavirus-induced host granulocytes apoptosis. Luo, Kaijun,Pang, Yi. 2006

[11]Molecular cloning, expression, and enzymatic activity of a novel endogenous cellulase from the mulberry longicorn beetle, Apriona germari. Wei, Ya Dong,Lee, Kwang Sik,Gui, Zhong Zheng,Yoon, Hyung Joo,Kim, Iksoo,Zhang, Guo Zheng,Guo, Xijie,Sohn, Hung Dae,Jin, Byung Rae.

[12]Cetyltriethylammonium bromide stimulating transcription of Bombyx mori nucleopolyhedrovirus gp64 gene promoter mediated by viral factors. Zhou, YJ,Yi, YZ,Zhang, ZF,He, JL,Zhang, YX.

[13]Spread of recombinant Autographa californica nucleopolyhedrovirus in various tissues of silkworm Bombyx mori determined by real-time PCR. Zhang, Yi,Tian, Baozhong,Guo, Tingqing,Wang, Jianyang,Wei, Zhenguo,Lu, Changde,Zhang, Yi,Xia, Huanzhang,Tian, Baozhong,Wei, Zhenguo,Wang, Shengpeng.

[14]Effects of cetyltriethylammonium bromide on the replication of Bombyx mori nucleopolyhedrovirus. Zhou, YJ,Zhang, ZF,He, JL,Zhang, YX.

[15]Screening of candidate proteins interacting with IE-2 of Bombyx mori nucleopolyhedrovirus. Wu, Yejun,Wu, Yu,Wu, Yan,Tang, Hui,Wu, Huiling,Wang, Wenbing,Zhang, Guozheng.

[16]N-linked glycosylation of a beetle (Apriona germari) cellulase Ag-EGase II is necessary for enzymatic activity. Wei, YD,Lee, KS,Gui, ZZ,Yoon, HJ,Kim, I,Je, YH,Lee, SM,Zhang, GZ,Guo, XJ,Sohn, HD,Jin, BR.

[17]Establishment and characterization of a novel cell line from midgut tissue of Helicoverpa armigera (Lepidoptera: Noctuidae). He, Fei,Yang, Yongbo,Peng, Rong,Yao, Hanchao,Peng, Jianxin,Hong, Huazhu,Liu, Kaiyu,Xiao, Yutao,Li, Xianchun.

[18]Promoter analysis of Bombyx mori nucleopolyhedrovirus Ubiquitin gene. Lin, Xu'ai,Chen, Yin,Yi, Yongzhu,Zhang, Zhifang,Lin, Xu'ai,Yan, Jie,Chen, Yin.

[19]Characterization of the Autographa californica Nucleopolyhedrovirus Ubiquitin Gene Promoter. Lin, Xu Ai,Chen, Yin,Yi, Yong Zhu,Zhang, Zhi Fang,Xu, Wei Hua.

[20]Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs. Cao, Yimei,Lu, Zengjun,Sun, Jiachuan,Bai, Xingwen,Sun, Pu,Bao, Huifang,Chen, Yingli,Guo, Jianhong,Li, Dong,Liu, Xiangtao,Liu, Zaixin.

作者其他论文 更多>>