Selenium promotes broiler myoblast proliferation through the ROS/PTEN/PI3K/ AKT signaling axis

文献类型： 外文期刊

第一作者： Wang, Shengchen

作者： Wang, Shengchen;Tian, Bing;Feng, Xinyu;Hu, Yangyang;Hu, Yun;Cui, Xiaoyan;Li, Tingting;Luo, Xugang;Zhang, Liyang;Zhang, Ziwei;Xu, Shiwen

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关键词： selenium; broiler myoblast; proliferation; ROS; PTEN/PI3K/AKT pathway

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 12 期

页码：

收录情况： SCI

摘要： Selenium (Se), an indispensable trace element in broiler chickens, is closely associated with the growth and development of skeletal muscles. However, the role of Se in the proliferation of broiler myoblasts and its specific biological mechanisms have not been elucidated. In the present study, an in vitro growth model of broiler pectoral myoblasts cultured with Se (Na2SeO3) for 24 h was established. Using light microscopy, Cell Counting Kit-8 (CCK-8) assay, and flow cytometry, we found that compared to the control (Con) group, Se supplemental level obviously promoted myoblast proliferation and prevented cell cycle arrest from the G1 phase to the S + G2 phase. Through intracellular reactive oxygen species (ROS) generation detection, western blotting, and quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR), the study showed that the reduced ROS production caused by Se supplementation significantly decreased PTEN expression and activated the PI3K/AKT signaling pathway in myoblasts, thereby promoting the P53/P21/CyclinD1regulated cell cycle progression, as well as the expression of proliferation-related myogenic regulatory factors (MRF). Our findings support the potential of Se to maintain the proliferative capacity of chicken myoblasts and emphasize the importance of Se intake in regulating skeletal muscle growth and development in poultry.

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