Over-expression of ThpI from Choristoneura fumiferana enhances tolerance to cold in Arabidopsis
文献类型: 外文期刊
第一作者: Zhu, Bo
作者: Zhu, Bo;Xiong, Ai-Sheng;Peng, Ri-He;Jin, Xiao-Fen;Yao, Quan-Hong;Zhu, Bo;Xu, Jing;Meng, Xiu-Rong
作者机构:
关键词: plant growth;cold tolerance;electrolyte leakage activity
期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Thermal hysteresis proteins (Thps) known as antifreeze proteins for their antifreeze activity, depress the freezing point of water below the melting point in many polar marine fishes, terrestrial arthropods and plants. For the purpose of breeding cold-resistant plants, we designed to introduce the Thp gene into the plants. The physiological and biochemical effect of high-lever expression of the modified Choristoneura fumiferana Thp (ThpI) in Arabidopsis thaliana plants was analyzed. Under low temperature stress, the ThpI transgenic plants exhibited stronger growth than wild-type plants. The elevated cold tolerance of the ThpI over-expressing plants was confirmed by the changes of electrolyte leakage activity, malonyldialdehyde and proline contents. These results preliminarily showed that the Thp possibly be used to enhance the low temperature-tolerant ability of plants.
分类号: Q7
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