Biocontrol Ability of Lysobacter antibioticus HS124 Against Phytophthora Blight Is Mediated by the Production of 4-Hydroxyphenylacetic Acid and Several Lytic Enzymes

文献类型： 外文期刊

第一作者： Ko, Hyun-Sun

作者： Ko, Hyun-Sun;Kim, Kil-Yong;Jin, Rong-De;Krishnan, Hari B.;Lee, Sang-Bog

作者机构：

关键词： enzyme activity

期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Several rhizobacteria play a vital role in plant protection, plant growth promotion and the improvement of soil health. In this study, we have isolated a strain of Lysobacter antibioticus HS124 from rhizosphere and demonstrate its antifungal activity against various pathogens including Phytophthora capsici, a destructive pathogen of pepper plants. L. antibioticus HS124 produced lytic enzymes such as chitinase, o-1,3-glucanase, lipase, protease, and an antibiotic compound. This antibiotic compound was purified by diaion HP-20, silica gel, sephadex LH-20 column chromatography and high performance liquid chromatography. The purified compound was identified as 4-hydroxyphenylacetic acid by gas chromatography-electron ionization (GC-EI) and gas chromatography-chemical ionization (GC-CI) mass spectrometry. This antibiotic exhibited destructive activity toward P. capsici hyphae. In vivo experiments utilizing green house grown pepper plants demonstrated the protective effect of L. antibioticus HS124 against P. capsici. The growth of pepper plants treated with L. antibioticus culture was enhanced, resulting in greater protection from fungal disease. Optimum growth and protection was found when cultures were grown in presence of Fe(III). Additionally, the activities of pathogenesis-related proteins such as chitinase and o-1,3-glucanase decreased in roots, but increased in leaves with time after treatment compared to controls. Our results demonstrate L. antibioticus HS124 as a promising candidate for biocontrol of P. capsici in pepper plants.

分类号： Q93

  • 相关文献

[1]Identification of a UDP-glucose pyrophosphorylase from cotton (Gossypium hirsutum L.) involved in cellulose biosynthesis in Arabidopsis thaliana. Qinghua Wang,Xue Zhang,Fuguang Li,Yuxia Hou,Xingliang Liu,Xueyan Zhang.

[2]Effects of Bacterial-Feeding Nematodes and Glucose on Phenanthrene Removal by Pseudomonas putida. Jing Yongping,Li Yan,Zhang Yingpeng,Liu Ping,Sun Ming,Jing Yongping,Li Yan,Zhang Yingpeng,Jing Yongping,Liu Zhaohui,Luo, Jiafa. 2017

[3]Response of the enzymes to nitrogen applications in cotton fiber (Gossypium hirsutum L.) and their relationships with fiber strength. Wang YouHua,Feng Ying,Xu NaiYin,Chen BingLin,Ma RongHui,Zhou ZhiGuo,Xu NaiYin. 2009

[4]Physiological and morphological responses induced by alpha-particle radiation on Arabidopsis thaliana embryos. Ren, J.,Liu, L.,Fu, S. L.,Ren, J.,Jin, X. L.,Ding, Z. C.,Liu, L.. 2014

[5]Canonical correspondence analysis of soil heavy metal pollution, microflora and enzyme activities in the Pb-Zn mine tailing dam collapse area of Sidi village, SW China. Li, Zhongyi,Li, Qiang,Hu, Qingjing,Jin, Zhenjiang,Li, Zhongyi,Li, Qiang,Hu, Qingjing,Jin, Zhenjiang,Yang, Rongmei,Tang, Huafeng,Li, Min,Huang, Bingfu,Zhang, Jiayu,Li, Guiwen,Li, Zhongyi,Hu, Qingjing. 2015

[6]Sucrose and citric acid accumulations in melon genotypes with different sugar and acid contents. Tang, Mi,Zhang, Bao-cai,Xie, Jun-jun,Bie, Zhi-long,Wu, Ming-zhu,Yi, Hong-ping,Feng, Jong-xin,Tang, Mi. 2012

[7]Molecular characterization of a cathepsin F-like protease in Trichinella spiralis. Qu, Zi-gang,Ma, Xue-ting,Li, Wen-hui,Zhang, Nian-zhang,Yue, Long,Cui, Jian-min,Cai, Jian-ping,Jia, Wan-zhong,Fu, Bao-quan,Cai, Jian-ping,Jia, Wan-zhong,Fu, Bao-quan. 2015

[8]Distinct responses of soil bacterial and fungal communities to changes in fertilization regime and crop rotation. Zhang, Shuiqing,Guo, Doudou,Huang, Shaomin,Ai, Chao,Zhang, Xin,Zhou, Wei. 2018

[9]Physical changes and physiological characteristics of red and green peel during nectarine (cv. Hu018) maturation. Su, Mingshen,Chen, Kunsong,Zhang, Bo,Xu, Changjie,Sun, Chongde,Zhang, Jiukai,Li, Xian,Xi, Wanpeng,Su, Mingshen,Ye, Zhengwen,Wu, Aizhong,Guo, Juan. 2012

[10]Molecular characterization, expression and activity of glucokinase in golden pompano, Trachinotus ovatus: Response of its expression to carbohydrate in the diet. Zhou, Chuanpeng,Wang, Jun,Wang, Yun,Lin, Heizhao,Huang, Zhong,Yu, Wei. 2018

[11]The effect of long-term reclamation on enzyme activities and microbial community structure of saline soil at Shangyu, China. Liu, Chen,Xu, JianMing,Liu, Chen,Ding, NengFei,Fu, QingLin,Guo, Bin,Lin, YiCheng,Li, Hua,Li, Ningyu. 2013

[12]Soil microbial communities and enzyme activities in a reclaimed coastal soil chronosequence under rice-barley cropping. Fu, Qinglin,Liu, Chen,Ding, Nengfei,Lin, Yicheng,Guo, Bin,Luo, Jiafa,Wang, Hailong. 2012

[13]Immunolocation and enzyme activity analysis of Cryptosporidium parvum enolase. Mi, Rongsheng,Chen, Zhaoguo,Mi, Rongsheng,Yang, Xiaojiao,Huang, Yan,Cheng, Long,Lu, Ke,Han, Xiangan,Chen, Zhaoguo,Mi, Rongsheng,Yang, Xiaojiao,Huang, Yan,Cheng, Long,Lu, Ke,Han, Xiangan,Chen, Zhaoguo. 2017

[14]EFFECTS OF VITRIFICATION PROTOCOL ON THE LACTATE DEHYDROGENASE AND TOTAL ATPASE ACTIVITIES OF CHINESE MITTEN CRAB Eriocheir sinensis EMBRYOS. Huang, Xiaorong,Feng, Guangpeng,Zhao, Feng,Liu, Jianyi,Zhang, Tao,Wang, Yu,Zhuang, Ping. 2016

[15]Accumulation, histopathological effects and response of biochemical markers in the spleens and head kidneys of common carp exposed to atrazine and chlorpyrifos. Wang, Xu,Xing, Houjuan,Jiang, Yan,Xu, Shiwen,Xing, Houjuan,Sun, Gang,Wu, Hongda,Xu, Qiyou. 2013

[16]Purification and characteristics of serine protease from the head of pacific white shrimp. Wu, Yanyan,Li, Laihao,Yang, Xianqing,Diao, Shiqiang,Wang, Ping. 2012

[17]Effect of high hydrostatic pressure on overall quality parameters of watermelon juice. Liu, Y.,Zhao, X. Y.,Hu, X. S.. 2013

[18]Characterization of the putative tryptophan synthase beta-subunit from Mycobacterium tuberculosis. Shen, Hongbo,Yang, Yanping,Wang, Feifei,Xu, Shengfeng,Wang, Honghai,Zhang, Ying,Ye, Naihao. 2009

[19]Physiological Basis of Improved Performance of Super Rice (Oryza sativa) to Deep Placed Fertilizer with Precision Hill-drilling Machine. Kargbo, M. B.,Pan, Shenggang,Mo, Zhaowen,Tian, Hua,Hossain, Md. Faruque,Ashraf, Umair,Tang, Xiangru,Pan, Shenggang,Mo, Zhaowen,Tian, Hua,Ashraf, Umair,Tang, Xiangru,Kargbo, M. B.,Wang, Zaiman,Luo, Xiwen. 2016

[20]Light-regulated phosphorylation of maize phosphoenolpyruvate carboxykinase plays a vital role in its activity. Chao, Qing,Mei, Ying-Chang,Gao, Zhi-Fang,Chen, Yi-Bo,Wang, Bai-Chen,Liu, Xiao-Yu,Qian, Chun-Rong,Hao, Yu-Bo. 2014

作者其他论文 更多>>

微信小程序