Effects of 5-aminolevulinic acid on the inflammatory responses and antioxidative capacity in broiler chickens challenged with lipopolysaccharide

文献类型： 外文期刊

第一作者： Chen, Jiang

作者： Chen, Jiang;Wang, Huakang;Wu, Zhengke;Gu, Hongxia;Li, Chong;Wang, Shaolong;Liu, Guohua

作者机构：

关键词： delta-Aminolevulinic acid; Haem oxygenase-1; Inflammation; Oxidative stress; TLR4/NF-kappa B

期刊名称：ANIMAL （ 影响因子：3.73； 五年影响因子：3.908 ）

ISSN： 1751-7311

年卷期： 2022 年 16 卷 7 期

页码：

收录情况： SCI

摘要： 5-Aminolevulinic acid (5-ALA) is an intermediate in haem biosynthesis and has anti-apoptotic, anti-inflammatory, antioxidant, and other pharmacological effects. This study aimed to investigate the effect of dietary supplementation with 5-ALA on growth performance, antioxidant capacity, and inflammatory response of the lipopolysaccharide (LPS)-challenged broiler chickens. The experiment was designed as a 2 x 2 factorial arrangement with dietary 5-ALA (0 or 60 mg/kg) and LPS (injection of saline or 0.5 mg/kg BW) levels as treatments. A total of 240 one-day-old Arbor Acres broilers were distributed into four treat-ments consisting of six replicates of 10 birds. All the experimental broilers were intraperitoneally injected with LPS or sterile saline at 16, 18, and 20 days of age. Our results showed that dietary 5-ALA supplemen-tation reduced (P < 0.05) the feed to gain before broilers were stimulated with LPS (days 1-15). LPS chal-lenge decreased (P < 0.05) the catalase (CAT), total superoxide dismutase activities and increased the content of malondialdehyde (MDA) in the serum of broiler chickens. However, 5-ALA supplementation had a tendency to increase (P = 0.08) the activity of CAT and decreased (P < 0.05) the content of MDA. LPS challenge showed higher (P < 0.05) interleukin (IL)-1B, IL-6, and IL-10 concentrations in the serum, whereas dietary 5-ALA supplementation decreased (P < 0.05) the levels of IL-1B and IL-6. Additionally, dietary 5-ALA supplementation significantly attenuated (P < 0.05) the upregulation of mRNA expression levels of hepatic toll-like receptor 4 (TLR4), IL-1B, and IL-2 induced by LPS challenge. Moreover, dietary 5 -ALA supplementation also enhanced the mRNA expression of 5-aminolevulinate dehydratase, fer-rochelatase, and haem oxygenase-1 (HO-1) as compared to the unsupplemented groups. In conclusion, our results suggested that supplementation of 60 mg/kg 5-ALA exhibited LPS-induced anti-inflammatory and antioxidant properties by enhancing the HO-1 expression and inhibiting the TLR4/ NF -KB signalling pathway. (C) 2022 The Authors. Published by Elsevier B.V. on behalf of The Animal Consortium.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序