Genome-Wide Protein Interaction Analysis in Parasitic Gyrodactylus Flatworms-Fish Hosts System and Drug Target Identification

文献类型： 外文期刊

第一作者： Zhang, Dong

作者： Zhang, Dong;Zhao, Jie-Mei;Xiang, Chuan-Yu;Ma, Yi-Wen;Shi, Yu-Ying;Hu, Ye;Zhang, Feng;Liu, Xiang;Jakovlic, Ivan;Zhang, Dong;Zhao, Jie-Mei;Xiang, Chuan-Yu;Ma, Yi-Wen;Shi, Yu-Ying;Hu, Ye;Zhang, Feng;Liu, Xiang;Jakovlic, Ivan;Zhang, Dong;Liu, Fei;Lei, Hong-Peng;Zeng, Xiaofei;Zhou, Shun;Chen, Jinsong;Chen, Jinsong;Liu, Fei;Zeng, Benhe;Song, Rui;Song, Rui;Li, Wen-Xiang;Wang, Gui-Tang

作者机构：

关键词： antagonistic coevolution; cAMP signaling; GPCRs; immune coadaptation; innexins; interactome; PRKACB

期刊名称：ADVANCED SCIENCE （ 影响因子：14.1； 五年影响因子：15.6 ）

ISSN：

年卷期： 2025 年

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收录情况： SCI

摘要： The host-parasite arms race involves complex molecular crosstalk mediated by protein-protein interactions (PPIs). Bioinformatic analyses can be used to predict both host-parasite PPIs and potential drug targets in parasite genomes, but high-quality genomic data remain scarce for parasitic monogenean flatworms. Herein, an experimental lineage of Gyrodactylus kobayashii (Monopisthocotylea: Gyrodactylidae) is set up on goldfish hosts and used to conduct phased genome assembly using long-range PacBio HiFi and Hi-C technologies. In silico analyses of genomes of three Gyrodactylus species identified innexins as the most promising novel drug candidate genes. Drug screening and experimental verification singled out Imatinib as the most promising drug targeting innexins, with a high efficiency against G. kobayashii (100% mortality at 25 mu M within 6 h in vitro) and low toxicity to the host. Prediction of PPIs in three Gyrodactylus-host pairs revealed proteins associated with cAMP-dependent signaling as key candidates, including the host's PRKACB and the parasite's PRKAR2A, RAP1A, ULK2, and Catenin Beta-2. Two interacting G proteins are also identified: GNAO1 and GNB5. As the first high-quality phased chromosome-level genomic assembly for "monogeneans" and the first identification of PPIs in a fish-parasite system, this study significantly advances the understanding of host-parasite interactions at the genomic level.

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