Folate metabolic profiling and expression of folate metabolism-related genes during panicle development in foxtail millet (Setaria italica (L.) P. Beauv)

文献类型： 外文期刊

第一作者： Hou, Siyu

作者： Hou, Siyu;Man, Xiaxia;Lian, Boying;Ma, Guifang;Sun, Zhaoxia;Yan, Lufei;Gao, Hao;Du, Wei;Wang, Xinfang;Li, Hongying;Han, Yuanhuai;Hou, Siyu;Sun, Zhaoxia;Li, Hongying;Han, Yuanhuai;Han, Lida;Zhang, Yijuan

作者机构：

关键词： folate metabolites; foxtail millet; folate metabolism pathway; gene expression; one-carbon metabolism

期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE （ 影响因子：3.639； 五年影响因子：3.803 ）

ISSN： 0022-5142

年卷期： 2022 年 102 卷 1 期

页码：

收录情况： SCI

摘要： BACKGROUND Foxtail millet grain has higher folate content than other cereal crops. However, the folate metabolite content and the expression patterns of folate metabolite-related genes are unknown. RESULTS Liquid chromatography-mass spectrometry was used to investigate 12 folate metabolites in a foxtail millet panicle. The content of total folate and derivatives gradually decreased during panicle development. Polyglutamate 5-formyl-tetrahydrofolate was the major form. Twenty-eight genes involved in the folate metabolic pathway were identified through bioinformatic analysis. These genes in Setaria italica, S. viridis and Zea mays showed genomic collinearity. Phylogenetic analysis revealed that the folate-related genes were closely related among the C4 plants compared to C3 plants. The gene expressions were then studied at three panicle development stages. The gene expression patterns were classified into two groups, namely SiADCL1 and SiGGH as two key enzymes, which are responsible for folate synthesis and degradation; their expression levels were highest at the early panicle development stage, up to 179.11- and 163.88-fold, respectively. Their expression levels had a similar downward trend during panicle development and were significantly positively correlated with the concentration of total folate and folate derivatives. However, SiSHMT3 expression levels were significantly negatively correlated with total folate concentration. CONCLUSION Besides being the major determinants of folate and folate derivatives accumulation, SiADCL1 and SiGGH expression levels are key limiting factors in the foxtail millet panicle. Therefore, SiADCL1 and SiGGH expression levels can be targeted in genetic modification studies to improve folate content in foxtail millet seeds in the future. (c) 2021 Society of Chemical Industry

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序