Hybrid nanoflower-based electrochemical lateral flow immunoassay for Escherichia coli O157 detection

文献类型： 外文期刊

第一作者： Wei, Jiaqi

作者： Wei, Jiaqi;Qu, Guijuan;Bu, Shengjun;Zhou, Hongyu;Sun, He;Hao, Zhuo;Wan, Jiayu;Bu, Shengjun

作者机构：

关键词： Lateral flow immunochromatography; Electrochemistry; Signal amplification; E. coli O157:H7; Visual detection

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：5.0 ）

ISSN： 0026-3672

年卷期： 2024 年 191 卷 8 期

页码：

收录情况： SCI

摘要： An electrochemical biosensor has been developed for detection of Escherichia coli O157 by integrating lateral flow with screen-printed electrodes. The screen-printed electrodes were attached under the lateral flow detection line, and organic-inorganic nanoflowers prepared from E. coli O157-specific antibodies as an organic component were attached to the lateral flow detection line. In the presence of E. coli O157, an organic-inorganic nanoflower-E. coli O157-antimicrobial peptide-labelled ferrocene sandwich structure is formed on the lateral flow detection line. Differential pulse voltammetry is applied using a smartphone-based device to monitor ferrocene on the detection line. The resulting electrochemical biosensor could specifically detect E. coli O157 with a limit of detection of 25 colony-forming units mL-1. Through substitution of antibodies of organic components in organic-inorganic nanoflowers, biosensors have great potential for the detection of other pathogens in biomedical research and clinical diagnosis.

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