In vivo protective effect of late embryogenesis abundant protein (ApSK(3) dehydrin) on Agapanthus praecox to promote post-cryopreservation survival

文献类型： 外文期刊

第一作者： Huang, Tingting

作者： Huang, Tingting;Sheng, Jiangyuan;Zhang, Di;Deng, Shan

作者机构：

关键词： Dehydrin; Embryogenic callus; Cryopreservation; RNAi; Gene overexpression

期刊名称：BIOCELL （ 影响因子：1.11； 五年影响因子：0.977 ）

ISSN： 0327-9545

年卷期： 2022 年 46 卷 11 期

页码：

收录情况： SCI

摘要： Dehydrins (DHNs), as members of the late embryogenesis abundant protein family, play critical roles in the protection of seeds from dehydration and plant adaptation to multiple abiotic stresses. Vitrification is a basic method in plant cryopreservation and is characterized by forming a glassy state to prevent lethal ice crystals produced during cryogenic storage. In this study, ApSK(3) type DHN was genetically transformed into embryogenic calluses (EC) of Agapanthus praecox by overexpression (OE) and RNA interference (RNAi) techniques to evaluate the in vivo protective effect of DHNs during cryopreservation. The cell viability showed a completely opposite trend in OE and RNAi cell lines, the cell relative death ratio was decreased by 20.0% in ApSK(3)-OE EC and significantly increased by 66.15% in ApSK(3)-RNAi cells after cryopreservation. Overexpression of ApSK(3) increased the content of non-enzymatic antioxidants (AsA and GSH) and up-regulated the expression of CAT, SOD, POD, and GPX genes, while ApSK(3)-RNAi cells decreased antioxidant enzyme activities and FeSOD, POD, and APX genes expression during cryopreservation. These findings suggest that ApSK(3) affects ROS metabolism through chelating metal ions (Cu2+ and Fe3+), alleviates H2O2 and OH center dot excessive generation, activates the antioxidant system, and improves cellular REDOX balance and membrane lipid peroxidation damage of plant cells during cryopreservation. DHNs can effectively improve cell stress tolerance and have great potential for in vivo or in vitro applications in plant cryopreservation.

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[1]Oxidative stress protective function of ApY(2)SK(2) dehydrin: a late embryogenesis abundant protein in embryogenic callus of Agapanthus praecox to promote post-cryopreservation survival. Liu, Tao,Zhang, Yiying,Chu, Yunxia,Chen, Hairong,Ren, Li,Liu, Tao,Zhang, Di,Chu, Yunxia. 2022

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