Illumina high-throughput sequencing for the genome of emerging fowl adenovirus D species and C species simultaneously

文献类型： 外文期刊

第一作者： Cui, Ning

作者： Cui, Ning;Sun, Shiping;Sun, Shouli;Xu, Chuantian;Hrabchenko, Nataliia;Huang, Qinghua;Cui, Ning;Sun, Shiping;Sun, Shouli;Xu, Chuantian;Hrabchenko, Nataliia;Huang, Qinghua;Sun, Shiping;Su, Shuai;Lu, Mei

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关键词： fowl adenovirus; mix infection; high-throughput; genome; evolution

期刊名称：POULTRY SCIENCE （ 影响因子：4.014； 五年影响因子：4.192 ）

ISSN： 0032-5791

年卷期： 2023 年 102 卷 1 期

页码：

收录情况： SCI

摘要： In recent years, clinical cases of inclusion body hepatitis (IBH) and hydropericardium syndrome (HPS) have been emerging and increasing in chicken flocks worldwide. Mixed infections with 2 or more fowl adenovirus (FAdV) serotypes were common in these cases. Herein, we collected a clinical sample that was and HPS symptoms in Shandong province of China and sample contained 2 FAdV strains of D species and C spewith a G+C content of 53.51%, whereas SD1763-2 strain was 43,721 nt in length, with a G+C content of 54.87%. Sequence alignment , phylogenetic analysis revealed that strain SD1763-1 was clustered together with sero-type 2/11 of FAdV-D , SD1763-2 was clustered together with FAdV-4. There is no recombination between the genomes of the 2 viruses of FAdV-D and FAdV-C in the present study. This is the first report of obtaining 2 genomic sequences of FAdV strains simulta-neously by direct use of deep sequencing in one clinical individual chicken sample, which provided direct evi-dence for mixed infections of adenovirus serotypes in the clinic and enriched the genome data to explore the geo-graphic biomarkers and virulence signatures of the genus Aviadenovirus.

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