Immediate, sensitive and specific time-resolved fluorescent immunoassay strips based on immune competition for the detection of procymidone in vegetables
文献类型： 外文期刊
第一作者： Li, Gaozhen
作者： Li, Gaozhen;Guan, Fukai;Xu, Huihui;Sun, Jiashuai;Huang, Jingcheng;Yang, Qingqing;Guo, Yanyin;Sun, Xia;Guo, Yemin;Li, Gaozhen;Guan, Fukai;Sun, Jiashuai;Huang, Jingcheng;Yang, Qingqing;Sun, Xia;Guo, Yemin;Li, Gaozhen;Guan, Fukai;Sun, Jiashuai;Huang, Jingcheng;Yang, Qingqing;Sun, Xia;Guo, Yemin;Zhao, Shancang
作者机构：
关键词： Procymidone; Vegetables; Time -resolved fluorescent immunoassay strip; Ultrahigh -performance liquid chromatography; tandem mass spectrometry
期刊名称：FOOD CONTROL （ 影响因子：6.0； 五年影响因子：5.8 ）
ISSN： 0956-7135
年卷期： 2023 年 147 卷
页码：
收录情况： SCI
摘要： As an organochlorine pesticide, procymidone has become an increasing serious problem in vegetables in recent years. In order to solve a series of problems such as low sensitivity of the immunological methods, a timeresolved fluorescent immunoassay strip (TRFIAS) was developed with low limit of detection (LOD). Based on the principle of antigen-antibody immune competition, TRFIAS could detect procymidone in vegetables. After the targets being added, the samples were incubated at 37 degrees C for 10 min. TRFIAS was observed under a UV lamp of 365 nm. The results were processed by ImageJ software to complete the qualitative and quantitative analysis of TRFIAS. Under the optimal working conditions, a linear range of TRFIAS was 5-250 ng/mL with a LOD of 0.33 ng/mL and a visual limit of detection (VDL) of 50 ng/mL. And the TRFIAS had good specificity, precision and accuracy. Comparing the results of TRFIAS with those of ultrahigh-performance liquid chromatographytandem mass spectrometry (UPLC-MS/MS), the two assays were in good agreement. Therefore, TRFIAS has the advantages of high sensitivity, high specificity, simple operation and relatively short assay time. Meanwhile, it shows absolutely great advantages in both quantitative and qualitative analysis.
分类号：
- 相关文献
作者其他论文 更多>>
-
Preparation of a novel ATP liposome and its regulation of postharvest senescence in Agaricus bisporus
作者：Jiang, Xin;Han, Jiali;Feng, Lei;Wang, Jia;Wang, Xiangyou;Guo, Yanyin;Li, Ling;Chen, Cunkun;Chen, Cunkun;Kitazawa, Hiroaki
关键词：Agaricus bisporus; ATP; Egg yolk lecithin; Liposome; Preservation
-
Reverse design of haptens based on antigen spatial conformation to prepare anti-capsaicinoids&gingerols antibodies for monitoring of gutter cooking oil
作者：Nie, Kunying;Zhang, Jiali;Xu, Haitao;Ren, Keyun;Yu, Chunlei;Li, Falan;Yang, Qingqing;Zhang, Qi;Nie, Kunying;Zhang, Jiali;Xu, Haitao;Ren, Keyun;Yu, Chunlei;Li, Falan;Yang, Qingqing;Nie, Kunying;Zhang, Jiali;Xu, Haitao;Ren, Keyun;Yu, Chunlei;Li, Falan;Yang, Qingqing;Zhang, Qi;Zhang, Qi
关键词：Gutter cooking oil; Broad-spectrum monoclonal antibodies; Molecular docking; Virtual screening
-
Characterization and mechanism of simultaneous degradation of aflatoxin B1 and zearalenone by an edible fungus of Agrocybe cylindracea GC-Ac2
作者：Guo, Changying;Fan, Lixia;Ning, Mingxiao;Zhang, Bingchun;Ren, Xianfeng;Guo, Changying;Fan, Lixia;Ning, Mingxiao;Zhang, Bingchun;Ren, Xianfeng;Yang, Qingqing
关键词：edible fungi; aflatoxin B1; zearalenone; manganese peroxidase; simultaneous degradation
-
Dual-aspect attention spatial-spectral transformer and hyperspectral imaging: A novel approach to detecting Aspergillus flavus contamination in peanut kernels
作者：Guo, Zhen;Zhang, Jing;Li, Shiling;Shao, Xijun;Dong, Haowei;Sun, Jiashuai;Geng, Lingjun;Guo, Yemin;Sun, Xia;Xia, Lianming;Darwish, Ibrahim A.;Guo, Zhen;Li, Shiling;Shao, Xijun;Dong, Haowei;Sun, Jiashuai;Geng, Lingjun;Guo, Yemin;Sun, Xia;Xia, Lianming;Guo, Zhen;Li, Shiling;Shao, Xijun;Dong, Haowei;Sun, Jiashuai;Geng, Lingjun;Guo, Yemin;Sun, Xia;Xia, Lianming;Wang, Haifang;Zhang, Qi;Darwish, Ibrahim A.
关键词：Attention fusion mechanisms; Deep Learning; Convolutional neural network; Graph convolutional network; Peanut kernels
-
Identification of Trichderma harzianum in postharvest Agaricus bisporus and a novel control approach using eucalyptus essential oil emulsion
作者：Han, Jiali;Jiang, Xin;Feng, Lei;Wang, Jia;Wang, Xiangyou;Guo, Yanyin;Li, Ling;Zhou, Qingxin;Kitazawa, Hiroaki
关键词：Agaricus bisporus; Antifungal mechanisms; Eucalyptus essential oil; Postharvest disease; Trichoderma harzianum species complex
-
Pharmacokinetics of Enrofloxacin in Plasma, Urine, and Feces of Donkey (Equus asinus) after a Single Intragastric Administration
作者：Yang, Bowen;Liu, Shijie;Qu, Honglei;Guo, Yanxin;Huang, Shimeng;Zhao, Lihong;Ma, Qiugang;Cheng, Jie;Qu, Honglei;Ji, Chuanliang;Wang, Yantao;Zhao, Shancang
关键词：enrofloxacin; ciprofloxacin; pharmacokinetics; donkey; urinary excretion
-
Uridine diphosphate glucosyltransferase is vital for fenpropathrin resistance in Bombyx mori (Lepidoptera)
作者：Zheng, Kai-yi;Zhang, Xiao-ying;Lisan, Fasihul;Lai, Wen-Qin;Zhang, Qiang;Lv, Jun-li;Lu, Zhan-peng;Qin, Sheng;Sun, Xia;Wang, Xue-yang;Li, Mu-wang;Qin, Sheng;Sun, Xia;Wang, Xue-yang;Li, Mu-wang;Zhang, Shang-zhi;Dai, Li-shang;Wang, Xue-yang;Li, Mu-wang;Dai, Li-shang
关键词：Bombyx mori; fenpropathrin; pest management; resistance mechanism; UGT
