Development and validation of an immunogold chromatographic test for on-farm detection of PRRSV

文献类型: 外文期刊

第一作者: Zhou, Sheng-hua

作者: Zhou, Sheng-hua;Cui, Shang-jin;Chen, Chang-mu;Zhang, Fan-chao;Li, Jun;Zhou, Shun

作者机构:

关键词: Immunochromatographic test strip;Porcine reproductive and respiratory syndrome virus

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

ISSN:

年卷期:

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收录情况: SCI

摘要: An immunochromatographic test strip was developed to detect porcine reproductive and respiratory syndrome virus (PRRSV). The test uses two gold-labeled monoclonal antibodies: D5 against recombinant nucleocapsid protein (rN) and E9 against recombinant M protein (rM). In the test, PRRSV binds to a mixture of D5 and E9 labeled with colloidal gold; the complexes move through a membrane and are captured by rabbit anti-rM and anti-rN antibodies at a test line, producing a reddish-purple band because of the increased concentration of gold. Unbound monoclonal antibodies move past the test line to be captured by goat anti-mouse antibodies, producing a band at a control line. In samples without PRRSV or with low virus concentration, a band appears only at the control line. A crossover-trial demonstrated that the test strip was highly specific for PRRSV. The test strip detection limit was between 7.8 x 10(3) and 1.6 x 10(4) TCID50/ml. Analysis of 100 clinical samples indicated that the sensitivity, specificity. and accuracy of the immunochromatographic test strip relative to reverse transcription polymerase chain reaction (RT-PCR) were 97.0, 93.9, and 96.0%. respectively. Because the test is simple and rapid, it can be used by an unskilled person to detect PRRSV in the field.

分类号: R37

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