Transcriptomic Study of Different Stages of Development in the Testis of Sheep

文献类型： 外文期刊

第一作者： Xi, Binpeng

作者： Xi, Binpeng;Zhang, Rui;Lu, Zengkui;Li, Jianye;An, Xuejiao;Yue, Yaojing;Xi, Binpeng;Zhang, Rui;Lu, Zengkui;Li, Jianye;An, Xuejiao;Yue, Yaojing;Xi, Binpeng;Zhao, Shengguo

作者机构：

关键词： F1 hybrid of Southdown x Hu sheep; transcriptomic study; development of testis; spermatogenesis

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 19 期

页码：

收录情况： SCI

摘要： Simple Summary The testis plays a crucial role in spermatogenesis and sperm production for the male sheep; the focus of this study was to use transcriptome to analyze the key developmental periods of an F1 hybrid of Southdown x Hu sheep, a cross between Southdown and Hu sheep. Through M0, M3 and M6 and Y1, four key-stage-two comparative screening differentially genes, and GO and KEGG enrichment analysis, we found the cAMP signaling pathway, MAPK signaling pathway, ECM-receptor interaction, PI3K-Akt signaling pathway, FOXO signaling pathway and other signaling pathways by enrichment, and south lake sheep testicular-development-related key genes have been found. These findings provide new insights into the development of sheep and help to improve sheep productivity.Abstract Numerous genes govern male reproduction, modulating testicular development and spermatogenesis. Our study leveraged RNA-Seq to explore candidate genes and pivotal pathways influencing fecundity in an F1 hybrid of Southdown x Hu sheep testes across four developmental milestones: M0 (0 months old, newborn), M3 (3 months old, sexually immature), M6 (6 months old, sexually mature), and Y1 (1 years old, adult). Histological examination using hematoxylins and eosin staining revealed that the cross-sectional area of the spermatid tubules and the number of supportive cells increased in the other groups, as compared to the M0 group. The cross-sectional area of the vasculature and the number of supporting cells were found to be significantly increased in all other groups in comparison to the M0 group. We conducted GO and KEGG analyses of the differentially expressed genes (DEGs) in the three comparison groups and identified key pathways, including cAMP, MAPK, ECM-receptor interactions, PI3K-Akt, and FOXO signaling, which are closely related to testicular development and spermatogenesis. Notably, alternative splicing (AS) events were markedly elevated in M6 and Y1 stages. Key genes like GATA4, GATA6, SMAD4, SOX9, YAP1, ITGB1 and MAPK1 emerged as significantly enriched in these pathways, potentially orchestrating the transition from immature to mature testes in sheep. These findings offer valuable insights into male reproductive potential and can inform strategies for optimizing animal breeding.

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