Overexpression of CYP6CX4 contributing to field-evolved resistance to flupyradifurone, one novel butenolide insecticide, in Bemisia tabaci from China

文献类型： 外文期刊

第一作者： Zhang, Qinghe

作者： Zhang, Qinghe;Tang, Juan;Gao, Bingli;Qu, Cheng;Luo, Chen;Wang, Ran;Tang, Juan;Wang, Jinda

作者机构：

关键词： Bemisia tabaci; Flupyradifurone; Cytochrome P450

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2024 年 265 卷

页码：

收录情况： SCI

摘要： Bemisia tabaci is a formidable insect pest worldwide, and exhibits significant resistance to various insecticides. Flupyradifurone is one novel butenolide insecticide and has emerged as a new weapon against B. tabaci , but fieldevolved resistance to this insecticide has become a widespread concern. To unravel the mechanisms of fieldevolved flupyradifurone resistance, we conducted a comprehensive investigation into susceptibility of twentyone field populations within the Beijing -Tianjin -Hebei Region of China. Alarmingly, thirteen of these populations displayed varying degrees of resistance, ranging from low to medium levels, and building upon our prior findings, we meticulously cloned and characterized the CYP6CX4 gene in B. tabaci . Our investigations unequivocally confirmed the association between CYP6CX4 overexpression and flupyradifurone resistance in three of the thirteen resistant strains via RNA interference. To further validate our findings, we introduced CYP6CX4 overexpression into a transgenic Drosophila melanogaster line, resulting in a significant development of resistance to flupyradifurone in D. melanogaster . Additionally, homology modeling and molecular docking analyses showed the stable binding of flupyradifurone to CYP6CX4 , with binding free energy of -6.72 kcal mol - 1 . Collectively, our findings indicate that the induction of CYP6CX4 exerts one important role in detoxification of flupyradifurone, thereby promoting development of resistance in B. tabaci.

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