Purification and characterization of the low molecular weight xylanase from Bacillus cereus L-1

文献类型： 外文期刊

第一作者： Zhang, Ge

作者： Zhang, Ge;Li, Zhihao;Chen, Guoqiang;Deng, Shuaijun;Zhang, Haibo;Li, Zhihao;Zhang, Liang;Cai, Wen;Wu, Lijun;Li, Hongtao;Liu, Haobao

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关键词： Bacillus cereus; Enzyme property; Low molecular weight; Production; Purification; Xylanase

期刊名称：BRAZILIAN JOURNAL OF MICROBIOLOGY （ 影响因子：2.2； 五年影响因子：2.8 ）

ISSN： 1517-8382

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Xylanase is widely used in various industries such as food processing, paper, textiles, and leather tanning. In this study, Bacillus cereus L-1 strain was isolated and identified as capable of producing low molecular weight xylanase through 16 s rRNA sequencing. Maximum xylanase yield of 15.51 +/- 2.08 U/mL was achieved under optimal fermentation conditions (5% inoculum, 20 g/L xylan, pH 6.0, for 24 h). After purification via ammonium sulfate precipitation and High-S ion exchange chromatography, electrophoretic purity xylanase was obtained with a 28-fold purification and specific activity of 244.97 U/mg. Xylanase had an optimal pH of 6.5 and temperature of 60 degrees C and displayed thermostability at 30 degrees C and 40 degrees C with 48.56% and 45.97% remaining activity after 180 min, respectively. The xylanase retained more than 82.97% of its activity after incubation for 24 h at pH 5.0 and was sensitive to metal ions, especially Mg2+ and Li+. Purified xylanase showed a molecular weight of 23 kDa on SDS-PAGE, and partial peptide sequencing revealed homology to the endo-1,4-beta-xylanase with a molecular weight of 23.3 kDa through LC/MS-MS (liquid chromatography-tandem mass spectrometry). This study suggests that the purified xylanase is easier to purify and enriches low molecular weight xylanases from bacteria source.

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