Ultrasensitive electrochemiluminescence aptasensor based on ABEI reduced silver nanoparticles for the detection of profenofos

文献类型： 外文期刊

第一作者： Han, Jie

作者： Han, Jie;Yu, Yanyang;Wang, Guanjie;Gao, Xiaolin;Geng, Lingjun;Sun, Jiashuai;Zhang, Mei;Meng, Xiaoya;Li, Falan;Sun, Xia;Guo, Yemin;Han, Jie;Yu, Yanyang;Wang, Guanjie;Gao, Xiaolin;Geng, Lingjun;Sun, Jiashuai;Zhang, Mei;Meng, Xiaoya;Li, Falan;Sun, Xia;Guo, Yemin;Han, Jie;Yu, Yanyang;Wang, Guanjie;Gao, Xiaolin;Geng, Lingjun;Sun, Jiashuai;Zhang, Mei;Meng, Xiaoya;Li, Falan;Sun, Xia;Guo, Yemin;Han, Jie;Shi, Ce;Ahmed, Mohamed Bedair Mohamed

作者机构：

关键词： Aptasensor; Electrochemiluminescence; ABEI-AgNPs-GO; Profenofos; Vegetables

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：10.753； 五年影响因子：10.237 ）

ISSN： 0048-9697

年卷期： 2022 年 844 卷

页码：

收录情况： SCI

摘要： An ultrasensitive electrochemiluminescence (ECL) aptasensor for detection of profenofos was constructed by the reducibility and chemiluminescence property of N-(aminobutyl)-N-(ethylisoluminol) (ABEI). ABEI was used to reduce silver nitrate (AgNO3) to silver nanoparticles (AgNPs), which could be adsorbed on the lattice of graphene oxide (GO) to form ABEI-AgNPs-GO complex. This compound could achieve excellent luminescence. The aptamer (Apt) modified (5 ') by sulfhydryl groups could be immobilized on AgNPs to capture profenofos. When profenofos was present, the ECL signal of the aptasensor would be weakened. To further demonstrate the successful construction of the aptasensor, cyclic voltammetry tests were performed on an electrochemical workstation and an ECL analyzer, respectively. The standard curve and specificity experiment both showed that the sensor had the advantages of low limit of detection (LOD) and good specificity. Under the optimal conditions, the aptasensor had a good linear response for profenofos in the range of 1 x 10(-1)-1 x 10(4) ng/mL. It also had a LOD of 6.7 x 10(-2) ng/mL and a correlation coefficient (R-2) of 0.9991. The aptasensor had been successfully applied to the detection of profenofos in vegetables. The recovery range of the proposed ECL aptasensor was 98 % similar to 107.4 %.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序