HSPC117 Improves the Rate of Embryonic Development by Up-Regulating the Transcription of Proto-Oncogenes

文献类型： 外文期刊

第一作者： Ma, Hong

作者： Ma, Hong;Fu, Bo;Wang, Liang;Li, Zhong-Qiu;Liu, Di

作者机构：

关键词： HSPC117; Porcine; Embryonic development; Proto-oncogenes; Epigenetics

期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）

ISSN： 0030-9923

年卷期： 2019 年 51 卷 5 期

页码：

收录情况： SCI

摘要： The developmental efficiency of cloned embryos from somatic cells remains low, and many factors contribute to overall development of the embryo, including gene expression by the donor cells. The human hematopoietic stem/progenitor cell 117 (HSPC117) protein has been identified as being involved in placental formation, and can be modified by epigenetics. However, whether HSPC117 affects development of cloned embryos mRNA expression is unknown. To investigate the influences of HSPC117 on embryonic development, we generated transgenic porcine embryos by handmade cloning. We then assessed the embryonic developmental rate at cleavage and blastocyst stages. Our results showed that the HSPC117 transgenic embryos had markedly higher cleavage and blastocyst rates when compared to the embryos with pcDNA 3.1 vector, (69.7 +/- 3.5% vs. 64.6 +/- 1.8%, and 24.8 +/- 2.2% vs. 15.9 +/- 4.3%, respectively). However, blastocyst cell number was not different between groups. Furthermore, proto-oncogene was reported to play roles in embryo development, thus we assessed c-Fos, c-Jun, Raf-1, and c-Myc mRNA expression in HSPC117 transgenic and pcDNA 3.1 blastocysts. It was revealed that over-expression of HSPC117 mRNA in blastocysts up-regulated c-Fos, c-Jun, Raf-1, and c-Myc mRNA expression. We suggest that over-expressed HSPC117 is an important contributing factor to the development of HMC embryos in vitro via the regulation of mRNA expression of several proto-oncogenes.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序