Functions of a Glucan Synthase Gene GFGLS in Mycelia! Growth and Polysaccharide Production of Grifola frondosa

文献类型： 外文期刊

第一作者： Cui, Feng-Jie

作者： Cui, Feng-Jie;Wu, Xi-Hong;Tao, Ting-Lei;Zan, Xin-Yi;Sun, Wen-Jing;Cui, Feng-Jie;Sun, Wen-Jing;Mu, Da-Shuai;Yang, Yan;Wu, Di

作者机构：

关键词： Grifola frondosa; glucan synthase; polysaccharide; gene silencing; RNA interference; mycelial growth

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2019 年 67 卷 32 期

页码：

收录情况： SCI

摘要： Glucan synthase (GLS) gene is known to be involved in the fungal biosynthesis of cell wall, differentiation, and growth. In the present study, a glucan synthase gene (GFGLS) in the edible mushroom Grifola frondosa with a full sequence of 5927 bp encoding a total of 1781 amino acids was cloned and characterized for the first time. GFGLSp_is a membrane.protein_ containing two large transmembrane domains connected with a hydrophilic cytoplasmic domain. With a constructed dual promoter RNA silencing vector pAN7-gfgls-dual, a GFGLS-silencing transformant iGFGLS-3 had the lowest GFGLS transcriptional expression level (26.1%) with a shorter length and thinner appearance of the mycelia, as well as decreased mycelial biomass and exo-polysaccharide production of 5.02 and 0.38 g/L, respectively. Further analysis indicated that GFGLS silence influenced slightly the monosaccharide compositions and ratios of mycelial and exo-polysaccharide. These findings suggest that GFGLS could affect mycelial growth and polysaccharide production by downregulating the glucan synthesis.

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