Optimization of an Ultrasound-Assisted Extraction for Simultaneous Determination of Antioxidants in Sesame with Response Surface Methodology

文献类型： 外文期刊

第一作者： Wang, Dandan

作者： Wang, Dandan;Zhang, Liangxiao;Xu, Yueqing;Qi, Xin;Wang, Xuefang;Wang, Xiupin;Zhang, Qi;Li, Peiwu;Wang, Dandan;Xu, Yueqing;Zhang, Liangxiao;Li, Peiwu;Zhang, Liangxiao;Qi, Xin;Wang, Xuefang;Wang, Xiupin;Li, Peiwu;Zhang, Qi;Li, Peiwu

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关键词： antioxidants; ultrasound-assisted extraction; liquid chromatography-tandem mass spectrometry (LC-MS; MS); sesame; simultaneous detection; response surface methodology

期刊名称：ANTIOXIDANTS （ 影响因子：6.312； 五年影响因子：6.648 ）

ISSN：

年卷期： 2019 年 8 卷 8 期

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收录情况： SCI

摘要： Sesame is a nutritional agricultural product with medicinal properties. Accurate determination of micronutrients is important for the improvement of sesame quality and nutrition assessments. Our previous study showed that 10 antioxidants-d-homoproline, vitamin B-2, coniferyl aldehyde, hesperidin, phloretin, N-acetyl-l-leucine, l-hyoscyamine, ferulic acid, 5-methoxypsoralen, and 8-methoxypsoralen-in sesame were potential characteristic nutrients in sesame. Herein, simultaneous detection of 10 different types of antioxidants was developed by using ultrasound-assisted extraction coupled with liquid chromatography-tandem mass spectrometry (UAE-LC-MS/MS) with the help of response surface methodology. The significant variables and levels were screened and optimized by combining the single factor experiment, Plackett-Burman test, and Box-Behnken design. The optimal conditions for extraction of target antioxidants in sesame were methanol solution of 75.0%, liquid-to-material ratio of 20:1 (mL/g), extraction temperature of 50 degrees C, extraction power of 410.0 W, extraction time of 65 min. The total yield of targets was 21.74 mu g/g under the optimized conditions. The mobile phase used was 0.1% formic acid in acetonitrile and 0.1% formic acid in water, and the column was a Thermo Syncronis C18 reverse phase column (100 mm x 2.1 mm, 3 mu m). All targets required only one injection and could be quickly separated and assayed within 7 min. The limits of detection and limits of quantification for these 10 nutritional compounds ranged from 0.01 to 0.11 mu g/kg and from 0.04 to 0.34 mu g/kg, respectively. The validation results indicated that the method had reasonable linearity (R-2 >= 0.9990), good recoveries (71.1%-118.3%), satisfactory intra-day precision (<= 9.6%) and inter-day precision (<= 12.9%), and negligible matrix effects (<= 13.8%). This simultaneous quantification method was accurate, fast, and robust for the assessment of sesame nutrition.

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