De novo transcriptome assembly and co-expression network analysis of Cynanchum thesioides: Identification of genes involved in resistance to drought stress

文献类型： 外文期刊

第一作者： Zhang, Xiaoyan

作者： Zhang, Xiaoyan;Yang, Zhongren;Zhang, Fenglan;Hao, Lizhen;Li, Zhi

作者机构：

关键词： Cynanchum thesioides (Freyn) K. Schum.; Cynanchum thesioides (Freyn) K. Schum. var. australe (maxim.) Tsiang et P. T. Li.; Drought resistance; Illumina sequencing; Differentially expressed genes; Weighted gene co-expression network

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2019 年 710 卷

页码：

收录情况： SCI

摘要： Cynanchum thesioides are upright, xerophytic shrubs that are widely distributed in arid and semi-arid areas of China, North Korea, Mongolia and Siberia. To date, little is known about the molecular mechanisms of drought resistance in C. thesioides. To better understand drought resistance, we used transcriptome analysis and Illumina sequencing technology on C. thesioides, to identify drought-responsive genes. Using de novo assembly 55,268 unigenes were identified from 207.58 Gb of clean data. Amongst these, 36,265 were annotated with gene descriptions, conserved domains, gene ontology terms and metabolic pathways. The sequencing results showed that genes that were differentially expressed (DEGs) under drought stress were enriched in pathways such as carbon metabolism, starch and sucrose metabolism, amino acid biosynthesis, phenylpropanoid biosynthesis and plant hormone signal transduction. Moreover, many functional genes were up-regulated under severe drought stress to enhance tolerance. Weighted gene co-expression network analysis showed that there were key hub genes related to drought stress. Hundreds of candidate genes were identified under severe drought stress, including transcriptional factors such as MYB, G2-like, ERF, C2H2, NAC, NF-X1, GRF, HD-ZIP, HB-other, HSF, C3H, GRAS, WRKY, bHLH and Trihelix. These data are a valuable resource for further investigation into the molecular mechanism for drought stress in C. thesioides and will facilitate exploration of drought resistance genes.

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