iTRAQ-based analysis of 17 beta-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis

文献类型： 外文期刊

第一作者： Zhu Ying

作者： Zhu Ying;Li Yangzhen;Li Hailong;Wang Lei;Zhang Ning;Liu Yang;Meng Liang;Xu Xiwen;Dong Zhongdian;Wei Min;Guo Hua;Cui Zhongkai;Li Xihong;Shao Changwei;Xu Wenteng;Zhu Ying;Li Yangzhen;Li Hailong;Wang Lei;Zhang Ning;Liu Yang;Meng Liang;Xu Xiwen;Dong Zhongdian;Wei Min;Guo Hua;Cui Zhongkai;Li Xihong;Shao Changwei;Xu Wenteng;Li Hailong

作者机构：

关键词： Cynoglossus semilaevis; 17 beta-estradiol; sex reversal; proteome; iTRAQ analysis

期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.265； 五年影响因子：1.276 ）

ISSN： 2096-5508

年卷期： 2019 年 37 卷 5 期

页码：

收录情况： SCI

摘要： The phenomenon of sex dimorphism prevails among many fish species. It has attracted the general research interest due to its close relationship to fish growth and thus aquaculture productivity. In Chinese tongue sole (Cynoglossus semilaevis), 17 beta-estradiol (E2) can be used reportedly to induce the feminization of fish, although the detailed regulatory network remained elusive. We treated the tongue sole fry before sex differentiation with E2 (10 and 30 mu g/L) to identify potential targets of E2 in Chinese tongue sole. The E2 treatment indeed induced genetic male fish sex-reversal to phenotypic female. Using an iTRAQ-based comparative proteomic analysis, 409 proteins that differentially expressed after E2 induction were identified, including 259 up-regulated and 150 down-regulated proteins. Furthermore, 19 differentially expressed proteins identified in the comparative proteomic analysis were selected to assess their transcription and eight of them exhibited the same tendency. Functions of the eight proteins included mainly nucleotide and protein binding. Interestingly, a far upstream element-binding protein 3-like isoform exhibited the significant upregulation both at transcription and translation levels after E2 treatment. This work identified a set of candidate proteins for E2 response and deepened our understanding of E2 regulatory mechanism.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序