Identification and Functional Analysis of the Pheromone Response Factor Gene of Sporisorium scitamineum

文献类型： 外文期刊

第一作者： Zhu, Guining

作者： Zhu, Guining;Deng, Yizhen;Cai, Enping;Yan, Meixin;Cui, Guobing;Zhang, Bin;Xi, Pinggen;Chang, Changqing;Jiang, Zide;Zhu, Guining;Yan, Meixin;Wang, Zhiqiang;Zou, Chengwu;Chen, Baoshan

作者机构：

关键词： Sporisorium scitamineum; pheromone response factor; mating; pathogenicity; fungi

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： The sugarcane smut fungus Sporisorium scitamineum is bipolar and produces sporidia of two different mating types. During infection, haploid cells of opposite mating types can fuse to form dikaryotic hyphae that can colonize plant tissue. Mating and filamentation are therefore essential for S. scitamineum pathogenesis. In this study, we obtained one T-DNA insertion mutant disrupted in the gene encoding the pheromone response factor (Prf1), hereinafter named SsPRF1, of S. scitamineum, via Agrobacterium tumefaciens-mediated transformation (ATMT) mutagenesis. Targeted deletion of SsPRF1 resulted in mutants with phenotypes similar to the T-DNA insertion mutant, including failure to mate with a compatible wild-type partner strain and being non-pathogenic on its host sugarcane. qRT-PCR analyses showed that SsPRF1 was essential for the transcription of pheromone-responsive mating type genes of the a1 locus. These results show that SsPRF1 is involved in mating and pathogenicity and plays a key role in pheromone signaling and filamentous growth in S. scitamineum.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序