SsCyp86 modulates sporisorium scitamineum mating/filamentation and pathogenicity through regulating fatty acid metabolism

文献类型： 外文期刊

第一作者： Cai, Enping

作者： Cai, Enping;Cai, Enping;Feng, Ruqing;Zheng, Wenqiang;Wang, Yifang;Chang, Changqing;Deng, Jiaru;Yan, Meixin

作者机构：

关键词： Sporisorium scitamineum; fatty acid metabolism; cytochrome P450 enzyme SsCyp86; mating/filamentation; pheromone response transcription factor SsPrf1

期刊名称：VIRULENCE （ 影响因子：5.5； 五年影响因子：5.4 ）

ISSN： 2150-5594

年卷期： 2024 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Background: Fatty acid metabolism constitutes a significant and intricate biochemical process within microorganisms. Cytochrome P450 (CYP450) enzymes are found in most organisms and occupy a pivotal position in the metabolism of fatty acids. However, the role of CYP450 enzyme mediated fatty acid metabolism in the pathogenicity of pathogenic fungi remains unclear. Methods: In this study, a CYP450 enzyme-encoding gene, SsCYP86, was identified in the sugarcane smut fungus Sporisorium scitamineum and its functions were characterized using a target gene homologous recombination strategy and metabonomics. Results: We found that the expression of SsCYP86 was induced by or sugarcane wax or under the condition of mating/filamentation. Sexual reproduction assay demonstrated that the SsCYP86 deletion mutant was defective in mating/filamentation and significantly reduced its pathogenicity. Further fatty acid metabolomic analysis unravelled the levels of fatty acid metabolites were reduced in the SsCYP86 deletion mutant. Exogenous addition of fatty acid metabolites cis-11-eicosenoic acid (C20:1N9), pentadecanoic acid (C15:0), and linolenic acid (C18:3N3) partially restored the mating/filamentation ability of the SsCYP86 deletion mutant and restored the transcriptional level of the SsPRF1, a pheromone response transcription factor that is typically down-regulated in the absence of SsCYP86. Moreover, the constitutive expression of SsPRF1 in the SsCYP86 deletion mutant restored its mating/filamentation. Conclusion: Our results indicated that SsCyp86 modulates the SsPRF1 transcription by fatty acid metabolism, and thereby regulate the sexual reproduction of S. scitamineum. These findings provide insights into how CYPs regulate sexual reproduction in S. scitamineum.

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