Development and application of sugarcane yellow leaf virus vectors in sugarcane
文献类型: 外文期刊
第一作者: Yin, Yuteng
作者: Yin, Yuteng;Zhao, Pingzhi;Sun, Yanwei;Han, Tian;Wang, Mengrong;Ye, Jian;Yin, Yuteng;Zhao, Pingzhi;Ye, Jian;Han, Tian;Wang, Mengrong;Yan, Meixin;Wu, Jianmin;Zhou, Hui
作者机构:
关键词: Sugarcane yellow leaf virus; Sugarcane; Aphid; Infectious clone; Expression vector
期刊名称:PHYTOPATHOLOGY RESEARCH ( 影响因子:3.5; 五年影响因子:3.9 )
ISSN: 2096-5362
年卷期: 2025 年 7 卷 1 期
页码:
收录情况: SCI
摘要: The aphid-transmitted polerovirus, sugarcane yellow leaf virus (SCYLV), is one of the most widespread sugarcane viruses and causes huge economic losses to the global sugarcane production. Molecular studies and disease resistance breeding against SCYLV are impeded both by the lack of infectious clones and comprehensive knowledge of this virus. In this study, we present the development of the first infectious cDNA clone of SCYLV, which could efficiently infect Nicotiana benthamiana, Arabidopsis thaliana and sugarcane plants. SCYLV-based recombinant heterologous EGFP protein vector was also developed. Using this system, we identified the green peach aphid, Myzus persicae, as a new compatible transmission vector and recognized LC05-136 and GT10-2118 as more resistant cultivars of SCYLV than Badila. This system provides an indispensable resource for both SCYLV biological research and sugarcane breeding efforts.
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