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Expression analysis and characterization of dmrt2 in Chinese tongue sole (Cynoglossus semilaevis)

文献类型：外文期刊

第一作者： Zhu, Ying

作者： Zhu, Ying;Cui, Zhongkai;Yang, Yingming;Xu, Wenteng;Shao, Changwei;Fu, Xiaoqin;Li, Yangzhen;Chen, Songlin;Zhu, Ying;Cui, Zhongkai;Yang, Yingming;Xu, Wenteng;Shao, Changwei;Fu, Xiaoqin;Li, Yangzhen;Chen, Songlin

作者机构：

关键词： dmrt2; Cynoglossus semilaevis; In situ hybridization; Gonadal differentiation/development; Male germ cell maturation

期刊名称：THERIOGENOLOGY （影响因子：2.74；五年影响因子：2.93 ）

ISSN： 0093-691X

年卷期： 2019 年 138 卷

页码：

收录情况： SCI

摘要： Dmrt2 is a member of the dmrt gene family with a conserved zinc finger-like DNA-binding motif (DM domain). In the present study, CS-dmrt2 was cloned from the gonads of Chinese tongue sole (Cynoglossus semilaevis). The full-length cDNA of CS-dmrt2 is 2834 bp in length, with a 251 bp 5'-untranslated region (UTR), a 1086 bp 3'-UTR and a 1503 bp open reading frame (ORF) that encodes a 501-amino-acid peptide. qPCR revealed that CS-dmrt2 was mainly expressed in C. semilaevis testes. In situ hybridization (ISH) showed CS-dmrt2 expression throughout early gonadal development (36 days after hatching (dah) and 86 dah), but the expression was higher in male gonads than in female gonads. CS-dmrt2 mRNA was highly expressed in male germ cells. Comparison of methylation levels between females and males demonstrated hypo-methylated levels of the CS-dmrt2 promoter in the male gonads, which is consistent with the high mRNA expression. These results suggest that the CS-dmrt2 gene may play a functional role in gonadal differentiation/development and germ cell maturation in the testis. (C) 2019 Published by Elsevier Inc.

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