Identification and expression profile of Delta-GST in black tiger shrimp (Penaeus monodon) exposed to aflatoxin B1 (AFB1)

文献类型： 外文期刊

第一作者： Wang, Yun

作者： Wang, Yun;Duan, Ya-Fei;Wang, Jun;Zhou, Chuan-Peng;Jiang, Shi-Gui;Lin, Hei-Zhao;Huang, Jian-Hua;Wang, Yun;Duan, Ya-Fei;Jiang, Shi-Gui

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关键词： aflatoxin B1; delta-class; glutathione S-transferase; penaeus monodon

期刊名称：JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY （ 影响因子：2.553； 五年影响因子：2.019 ）

ISSN： 2471-5638

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收录情况： SCI

摘要： Delta GST is an insect-specific class and a prominent class of the glutathione S-transferases family that is involved in xenobiotic detoxification and antioxidant defense. The full-length complementary DNA of delta-class GST from Penaeus monodon (PmDeltaGST; 839 bp long with a 657 bp coding region) was cloned. The encoded polypeptide of 218 amino acids had a predicted molecular mass of 24.30 kDa. Sequence homology and phylogenetic analysis showed that PmDeltaGST was significant similarity to GST genes in crustaceans and insects. Tissue expression profile analysis by quantitative real-time reverse-transcription polymerase chain showed that PmDeltaGST was constitutively expressed in all the examined tissues, with the highest expression in hepatopancreas and intestine and the weakest expression in ovary. PmDeltaGST messenger RNA expression and protein levels in hepatopancreas was significantly increased at 14 days postexposure of aflatoxin B1 (AFB1), keeping on the high level at 28 days, but decreased at 56 days. The results suggested that PmDeltaGST was involved in the response to AFB1 exposure.

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[1]Identification and expression profile of Delta-GST in black tiger shrimp (Penaeus monodon) exposed to aflatoxin B1 (AFB1). Wang, Yun,Duan, Ya-Fei,Wang, Jun,Zhou, Chuan-Peng,Jiang, Shi-Gui,Lin, Hei-Zhao,Huang, Jian-Hua,Wang, Yun,Duan, Ya-Fei,Jiang, Shi-Gui. 2019

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