A reliable and cost-efficient TLC-HPLC method for determining total florfenicol residues in porcine edible tissues

文献类型： 外文期刊

第一作者： Zhou, DanNa

作者： Zhou, DanNa;Li, YaChao;Sun, GuiZhi;Yang, Bo;Li, YaChao;Huang, LingLi;Qian, MingRong;Li, Dong

作者机构：

关键词： Total florfenicol residues; Florfenicol amine; High-performance liquid chromatography; Thin layer chromatography; Porcine edible tissues

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2020 年 303 卷

页码：

收录情况： SCI

摘要： It is still a challenge to solve the matrix interferences in veterinary drug residue analysis. In this study, we reported a thin layer chromatography (TLC)-high-performance liquid chromatography (HPLC) method for determining total florfenicol (FF) residues, expressed as florfenicol amine (FFA), in porcine edible tissues. The tissue homogenate were acid-hydrolyzed to liberate the bound residues and convert them into FFA. The hydrolysates were washed with ethyl acetate and subsequently extracted with ethyl acetate under alkaline conditions. The supernatants were concentrated through evaporation, defatted with hexane, purified by TLC and analyzed by HPLC at 225 nm. The optimal developing solvent for TLC purification was ethyl acetate-acetone-ammonium hydroxide mixtures (2:8:0.5, v/v/v). The method was fully validated according to decision 2002/657/EC, and could be used for the routine monitoring of FF residues in pig. TLC showed excellent purification efficiency, and was expected to solve the matrix interferences in veterinary drug residue analysis.

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