Functional characterization of PaCYP724B1 involved in shoot branching in sweet cherry (Prunus avium L.)

文献类型： 外文期刊

第一作者： Qi, Xiliang

作者： Qi, Xiliang;Liu, Congli;Song, Lulu;Li, Ming;Qin, Tengfei

作者机构：

关键词： PaCYP724B1; Shoot branching; Prunus avium; Overexpressing; Slencing

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2019 年 258 卷

页码：

收录情况： SCI

摘要： Sweet cherry (Prunus avium L.) is an economically important fruit crop, and has a variety of health promoting benefits. Because of sweet cherry tree possessing strong apical dominance, shoot branching, as a significant agriculture trait, determines fruit tree architecture and occupies an important position in sweet cherry yield. However, the mechanism that regulated sweet cherry shoot branching remains largely unknown. Here, we isolated and functionally characterized a sweet cherry gene homologous to the rice GNS4 gene, named PaCYP724B1. Spatial-temporal expression analysis revealed that PaCYP724B1 was expressed with a particularly high expression in the axillary shoots tissues during the shoot branching formation. Furthermore, overexpression of PaCYP724B1 in transgenic Arabidopsis plants enhanced shoot branching. In contrast, Silencing of NbCYP724B1 in Nicotiana tabacum via virus-induced gene silencing technology with heterologous PaCYP724B1 gene fragment resulted in a significantly inhibited shoot branching phenotype. Subcellular localization assays demonstrated that PaCYP724B1 protein was localized on the endoplasmic reticulum (ER). Taken together, the results denote new insights into the important role of PaCYP724B1 regulating sweet cherry shoot branching.

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