Small Grain and Dwarf 2, encoding an HD-Zip II family transcription factor, regulates plant development by modulating gibberellin biosynthesis in rice

文献类型： 外文期刊

第一作者： Chen, Weiwei

作者： Chen, Weiwei;Cheng, Zhijun;Wang, Min;Wang, Jian;Zhang, Feng;Zhang, Zhe;Wang, Yupeng;Luo, Sheng;Zhang, Jinhui;Wang, Jiulin;Wang, Jie;Zhao, Zhichao;Guo, Xiuping;Lei, Cailin;Zhang, Xin;Lin, Qibing;Ren, Yulong;Zhu, Shanshan;Wan, Jianmin;Liu, Linglong;You, Xiaoman;Zhou, Chunlei;Zhang, Huan;You, Shimin;Wan, Jianmin

作者机构：

关键词： Dwarfism; Small grain; GA biosynthesis; HD-Zip transcription factor; Oryza sativa

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2019 年 288 卷

页码：

收录情况： SCI

摘要： Homeodomain leucine zipper (HD-Zip) proteins are transcription factors that regulate plant development. Bioactive gibberellin (GA) is a key endogenous hormone that participates in plant growth. However, the relationship between HD-Zip genes and modulation of GA biosynthesis in rice remains elusive. Here, we identified a rice mutant, designated as small grain and dwarf 2 (sgd2), which had reduced height and grain size compared with the wild type. Cytological observations indicated that the defective phenotype was mainly due to decreased cell length. Map-based cloning and complementation tests demonstrated that a 9 bp deletion in a homeodomain leucine zipper (HD-Zip) II family transcription factor was responsible for the sgd2 mutant phenotype. Expression of SGD2 was pronounced in developing panicles, and its protein was localized in nucleus. Luciferase reporter system and transactivation assays in yeast suggested that SGD2 functioned as a transcriptional repressor. High performance liquid chromatography assays showed that the endogenous GA(1) level in the sgd2 mutant was dramatically decreased, and exogenous GA(3) recovered the second leaf sheath to normal length. Results of qRT-PCR showed that the expression levels of genes positively regulating GA-biosynthesis were mostly down-regulated in the mutant. Our data identified the role of an HD-Zip transcription factor that affects rice plant development by modulating gibberellin biosynthesis.

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