PREPARATION AND APPLICATION OF MONOCLONAL ANTIBODY TO SUGARCANE (SACCHARUM L. SPP. HYBRIDS) SOP5CS PROTEIN
文献类型： 外文期刊
第一作者： Li, J.
作者： Li, J.;Wang, L. R.;Xing, Y. X.;Yang, L. T.;Huang, X.;Li, Y. R.;Huang, X.;Li, Y. R.;Huang, X.;Li, Y. R.
作者机构：
关键词： sugarcane; P5CS gene; proline; monoclonal antibody; physiological index
期刊名称：APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH （ 影响因子：0.711； 五年影响因子：0.796 ）
ISSN： 1589-1623
年卷期： 2019 年 17 卷 5 期
页码：
收录情况： SCI
摘要： Delta(1)-pyrroline-5-carboxylate synthase (P5CS) is a key enzyme in proline biosynthesis. In this study, the nucleotide sequence of sugarcane P5CS (SoP5CS) gene was obtained from sugarcane. Then the ORF of SoP5CS linked with pET-30a vector and the SoP5CS protein was expressed successfully in E. coli by the induction of 1.0 mM IPTG at 28 degrees C for 6 h. The prokaryotic expression protein was expressed as inclusion bodies (IBs). It was purified by Ni-NTA Resin firstly and then refolded, dialyzed and further purified by gel filtration. The monoclonal antibody of SoP5CS was prepared using mice. The expression of SoP5CS gene and the corresponding protein and physiological parameters associated with drought resistance were detected in sugarcane leaves under different drought stresses, they worked together to regulate the drought resistance process of sugarcane. This is the first report on the preparation of P5CS monoclonal antibody and the expression of the antibody in sugarcane, which would provide new methods to study the function of SoP5CS response to various abiotic stresses and other aspects.
分类号：
- 相关文献
作者其他论文 更多>>
-
Metabolite analysis of peach (Prunus persica L. Batsch) branches in response to freezing stress
作者：Li, Y.;Wang, Y.;Wang, Z.;Liu, G.;Chang, R.;Chen, H.;Li, J.;Tian, Q.
关键词：Freezing stress; Metabolic networks; Metabolism; Peach
-
Effects of complex probiotics on intestinal function and its regulatory mechanism in patients with constipation
作者：Zhang, X.;Li, X.;Wang, X.;Li, J.;An, Y.;Li, L.;Zhang, P.;Dong, X.;Ze, X.
关键词：
-
MICROBIAL COMMUNITY STRUCTURE AND ITS DRIVING FACTORS IN THE WHITELEG SHRIMP ( LITOPENAEUS VANNAMEI) AQUACULTURE SYSTEM
作者：Qiao, L.;Bao, J. J.;Li, T. J.;Sun, X. M.;Ze, W. G.;Li, J.
关键词：aquaculture; shrimp; bacterial community; high-throughput sequencing; seawater
-
Effects of Biochar and α-Fe2O3 Nanoparticles on the Growth, Photosynthetic Apparatus and Fruit Quality of Medicinal Plant Muskmelon
作者：Wang, Y.;Zhou, W.;Yi, L.;Dai, Z.;Qiu, L.;Zhou, Y.;Wang, S.;Li, H.;Li, J.;Wang, Y.;Zhou, W.;Yi, L.;Dai, Z.
关键词：Cucumis melo; alpha-Fe2O3 nanoparticles; biochar; medicinal ingredients; muskmelon; fruit quality
-
The Complete Mitochondrial Genome of the Chinese Strain of Haemonchus contortus ChinHco
作者：Li, J. Y.;Gong, G. W.;Wang, F. W.;Pei, L.;Li, Y. N.;Zhu, C. X.;Luo, X. P.;Lai, J.;Xu, P. W.;Zhao, X. C.;Liu, Y. F.;Fan, F. J.;Li, J.;Li, J.
关键词：H. contortus; mitochondrial genome; nanopore sequencing; SURFSeq 5000; Chinese Strain ChinHco
-
Genome-wide association study identifies multiple loci influencing duck serum biochemical indicators in the laying period
作者：Qi, J.;Li, J.;Liu, H.;Zhou, Z.;Jiang, Y.;Fan, W.;Hu, J.;Guo, Z.;Xie, M.;Huang, W.;Zhang, Q.;Hou, S.;Liu, H.;Zhou, Z.;Jiang, Y.;Fan, W.;Hu, J.;Guo, Z.;Xie, M.;Huang, W.;Zhang, Q.;Hou, S.;Liu, H.;Zhou, Z.;Jiang, Y.;Fan, W.;Hu, J.;Guo, Z.;Xie, M.;Huang, W.;Zhang, Q.;Hou, S.;Hou, S.
关键词：Serum; biochemical indicator; laying period; GWAS; ducks
-
Organic selenium supplementation increases serum selenium levels in healthy Xinjiang brown cattle fed selenised yeast
作者：Li, Z.;Tang, J.;Li, J.;Ling, D.;He, X.;Tang, Y.;Yi, P.;Yang, Y.;Li, Z.;Li, J.;He, X.;Tang, Y.;Yi, P.;Tang, J.;Khoo, H. E.;Liu, Y.
关键词：blood metabolites; Bos taurus; enzyme activity; red dates; slaughter performance
