Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole

文献类型： 外文期刊

第一作者： Xu, Hao

作者： Xu, Hao;Xu, Xiwen;Li, Xihong;Wang, Lei;Zhou, Qian;Chen, Songlin;Xu, Hao;Xu, Xiwen;Li, Xihong;Wang, Lei;Zhou, Qian;Chen, Songlin;Xu, Hao;Cheng, Jiayu

作者机构：

期刊名称：SCIENTIFIC DATA （ 影响因子：6.444； 五年影响因子：9.051 ）

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年卷期： 2019 年 6 卷

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收录情况： SCI

摘要： Vibrio harveyi is a major bacterial pathogen that causes fatal vibriosis in Chinese tongue sole (Cynoglossus semilaevis), resulting in massive mortality in the farming industry. However, the molecular mechanisms of C. semilaevis response to V. harveyi infection are poorly understood. Here, we performed transcriptomic analysis of C. semilaevis, comparing resistant and susceptible families in response to V. harveyi challenge (CsRC and CsSC) and control conditions (CsRU and CsSU). RNA libraries were constructed using 12 RNA samples isolated from three biological replicates of the four groups. We performed transcriptome sequencing on an Illumina HiSeq platform, and generating a total of 1,095 million paired-end reads, with the number of clean reads per library ranging from 75.27 M to 99.97 M. Through pairwise comparisons among the four groups, we identified 713 genes exhibiting significant differences at the transcript level. Furthermore, the expression levels were validated by real-time qPCR. Our results provide a valuable resource and new insights into the immune response to V. harveyi infection.

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