文献类型： 外文期刊
第一作者： Zou, Ya-Jie
作者： Zou, Ya-Jie;Zhang, Jin-Xia;Wang, He-Xiang
作者机构：
关键词： Peroxidase; Mushroom; Pleurotus pulmonarius; Isolation
期刊名称：INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS （ 影响因子：1.931； 五年影响因子：1.712 ）
ISSN： 1573-3149
年卷期： 2019 年 25 卷 4 期
页码：
收录情况： SCI
摘要： A novel peroxidase has been isolated from fresh fruiting bodies of the mushroom Pleurotus pulmonarius, with a purification protocol of ion exchange chromatography on DEAE-cellulose, affinity chromatography on ConA-Sepharose, ion exchange chromatography on CM-cellulose, and gel filtration by FPLC on Superdex 75. The peroxidase was unadsorbed on either DEAE-cellulose or ConA-Sepharose, but was adsorbed on CM-cellulose. It exhibited a molecular mass of 55 kDa in gel filtration and also in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, indicating that it is a monomeric protein. It possessed a distinctive N-terminal amino acid sequences from other isolated mushroom peroxidases. The optimal pH and temperature for the enzyme were 4.0 and 70 degrees C, respectively. All enzyme activity was destroyed after exposured in 100 degrees C for 10 min. The peroxidase did not exhibit HIV-1 reverse transcriptase inhibitory activity and antifungal activity. The stability against high temperature and extreme pH supported that the enzyme could be a potential peroxidase source for special industrial applications.
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