Purification and characterization of a novel laccase from the edible mushroom Hericium coralloides

文献类型： 外文期刊

第一作者： Zou, Ya-Jie

作者： Zou, Ya-Jie;Huang, Chen-Yang;Zhang, Jin-Xia;Wang, He-Xiang;Ng, Tzi-Bun

作者机构：

关键词： Hericium coralloides;laccase;mushroom;purification

期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A novel laccase from the edible mushroom Hericium coralloides was purified by ion exchange chromatography on diethylaminoethyl (DEAE) cellulose, carboxymethyl (CM) cellulose, and Q-Sepharose columns followed by fast protein liquid chromatography gel filtration on a Superdex 75 column. Analysis by gel filtration and SDS-PAGE indicated that the protein is a monomer in solution with a molecular mass of 65 kDa. Its N-terminal amino acid sequence was AVGDDTPQLY, which exhibits partial sequence homology to previously isolated laccases. Optimum activity was observed at pH 2.2 and at 40°C. The enzyme showed activity toward a variety of substrates, the most sensitive of which was 2,2′-azinobis [3-ethylbenzothiazolone-6-sulfonic acid] diammonium salt (ABTS). The degradation activity toward substrates was ABTS > N,N-dimethyl-1,4-phenylenediamine > catechol > 2-methylcatechol > pyrogallol. The laccase did not exert any antiproliferative activity against Hep G2 or MCF 7 tumor cell lines at a concentration of 60 μM, unlike some previously reported mushroom proteins, but showed significant activity toward human immunodeficiency virus-1 (HIV-1) reverse transcriptase with an IC _(50) of 0.06 μM.

分类号： Q93

  • 相关文献

[1]A novel laccase with inhibitory activity towards HIV-I reverse transcriptase and antiproliferative effects on tumor cells from the fermentation broth of mushroom Pleurotus cornucopiae. Wu, Xiangli,Huang, Chenyang,Chen, Qiang,Zhang, Jinxia,Wu, Xiangli,Wang, Hexiang,Wu, Xiangli,Wang, Hexiang.

[2]First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha. Ma, Duanzheng,Wang, Hexiang,Sekete, Malota,Ma, Duanzheng,Wang, Hexiang,Wang, Bo,Ng, Tzibun. 2012

[3]An acid-tolerant lectin coupled with high Hg2+ potentiated hemagglutination enhancing property purified from Amanita hemibapha var. ochracea. Ma, Duanzheng,Wang, He Xiang,Sekete, Malota,Ma, Duanzheng,Wang, He Xiang,Sekete, Malota,Wang, Bo,Gong, Zhiyuan,Ng, Tzi Bun. 2014

[4]Cordysobin, a novel alkaline serine protease with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Cordyceps sobolifera. Wang, Shou-Xian,Wang, He-Xiang,Wang, Shou-Xian,Wang, He-Xiang,Wang, Shou-Xian,Liu, Yu,Zhao, Shuang,Xu, Feng,Geng, Xiao-Li,Zhang, Guo-Qing.

[5]Purification and Characterization of a Novel Lectin with Antiphytovirus Activities from the wild Mushroom Paxillus involutus. Wang, Shou Xian,Zhao, Shuang,Xu, Feng,Zhou, Ying,Geng, Xiao Li,Liu, Yu,Wang, Shou Xian,Wang, He Xiang,Wang, Shou Xian,Wang, He Xiang,Zhang, Guo Qing.

[6]A novel alkaline protease with antiproliferative activity from fresh fruiting bodies of the toxic wild mushroom Amanita farinosa. Sun, Jian,Wang, Hexiang,Sun, Jian,Wang, Hexiang,Zhao, Yongchang,Chai, Hongmei,Ng, Tzi Bun.

[7]Schizolysin, a hemolysin from the split gill mushroom Schizophyllum commune. Ng, Tzi Bun,Han, Chun-Hua,Han, Chun-Hua,Zhang, Guo-Qing,Wang, He-Xiang.

[8]A novel ribonuclease with potent HIV-1 reverse transcriptase inhibitory activity from cultured mushroom Schizophyllum commune. Zhang, Guo-Qing,Wang, He-Xiang,Zhang, Guo-Qing,Wang, He-Xiang,Zhao, Yong-Chang,Zhang, Guo-Qing,Ng, Tzi-Bun.

[9]Purification and characterization of a laccase from Coprinopsis cinerea in Pichia pastoris. Wang, Bo,Wang, Lijuan,Lin, Yaqiu,Han, Jing,Gao, Jianjie,Tian, Yongsheng,Zhao, Wei,Peng, Rihe,Yao, Quanhong,Wang, Bo,Wang, Lijuan,Han, Jing,Gao, Jianjie,Tian, Yongsheng,Zhao, Wei,Peng, Rihe,Yao, Quanhong,Lin, Yaqiu,Han, Qing,Han, Qing.

[10]Purification and characterization of a novel laccase from Coprinus cinereus and decolorization of different chemically dyes. Lin, Yaqiu,Zhang, Zhen,Xu, Zhisheng,Yao, Quanhong,Lin, Yaqiu,Tian, Yongsheng,Zhao, Wei,Zhu, Bo,Peng, Rihe,Yao, Quanhong.

[11]Purification and characterization of a novel laccase from the mushroom pleurotus nebrodensis. Wang, He-Xiang,Wang, He-Xiang,Ng, Tzi Bun,Zhang, Guo-Qing,Tian, Guo-Ting.

[12]Circular Multi-Storied Culture Mode among Strawberry, Mushroom and Leaf Vegetables in Greenhouse. Zhong, Chuanfei,Zhang, Yuntao,Wang, Guixia,Dong, Jing,Chang, Linlin,Wang, Lina. 2011

[13]Inhibiting Effect of Bioactive Metabolites Produced by Mushroom Cultivation on Bacterial Quorum Sensing-Regulated Behaviors. Zhu, Hu,Zhu, Hu,Zhang, Shuai-shuai,Cao, Chun-xu,Wang, Shou-xian. 2011

[14]Isolation and characterization of a novel thermostable lectin from the wild edible mushroom Agaricus arvensis. Zhao, J. K.,Wang, H. X.,Zhao, J. K.,Wang, H. X.,Zhao, Y. C.,Li, S. H.,Ng, T. B.. 2011

[15]Antioxidant Properties of Different Solvents Extracts from Three Edible Mushrooms. Lv, Guoying,Zhang, Zuofa,Pan, Huijuan,Fan, Leifa. 2009

[16]The genetic structure of the A mating-type locus of Lentinula edodes. Au, Chun Hang,Wong, Man Chun,Law, Patrick Tik Wan,Kwan, Hoi Shan,Bao, Dapeng,Zhang, Meiyan,Song, Chunyan,Song, Wenhua,Kues, Ursula.

[17]Development and characterization of simple sequence repeat (SSR) markers for the mushroom Flammulina velutipes. Zhang, Ruiying,Wang, Hexiang,Zhang, Ruiying,Wang, Hexiang,Zhang, Ruiying,Zhang, Jinxia,Zuo, Xuemei,Jiang, Ruibo,Hu, Dandan,Ng, Tzi Bun.

[18]A Fungal alpha-Galactosidase from Tricholoma matsutake with Broad Substrate Specificity and Good Hydrolytic Activity on Raffinose Family Oligosaccharides. Geng, Xueran,Wang, Hexiang,Tian, Guoting,Zhao, Yongchang,Zhao, Liyan,Ng, Tzi Bun.

[19]Cultivation and bioactivity assessment of Grifola frondosa fruiting bodies on olive oil press cakes substrates. Gregori, Andrej,Klinar, Dusan,Svagelj, Mirjan,Berovic, Marin,Liu, Yanfang,Zhang, Jingsong,Pohleven, Franc.

[20]Development of SSR markers for typing cultivars in the mushroom Auricularia auricula-judae. Zhang, Rui Ying,Gu, Jin Gang,Hu, Qing Xiu,Zuo, Xue Mei,Zhang, Rui Ying,Gu, Jin Gang,Hu, Qing Xiu,Zuo, Xue Mei,Hu, Dan Dan,Wang, He Xiang,Wang, He Xiang. 2012

作者其他论文 更多>>

微信小程序