Zinc finger antiviral protein (ZAP) inhibits small ruminant morbillivirus replication in vitro

文献类型： 外文期刊

第一作者： Tang, Aoxing

作者： Tang, Aoxing;Tang, Jingyu;Miao, Qiuhong;Zhu, Jie;Guo, Hongyuan;Liu, Chuncao;Meng, Chunchun;Li, Chuanfeng;Chen, Zongyan;Liu, Guangqing

作者机构：

关键词： Small ruminant morbillivirus (SRMV); Zinc finger antiviral protein (ZAP); Replication; ZRE

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2021 年 260 卷

页码：

收录情况： SCI

摘要： Small ruminant morbillivirus (SRMV) is a highly contagious and economically important viral disease of small domestic and wild ruminants. Difficulty with its stable proliferation in ovis aries-derived cells has led to a relative lag in the study of its natural immunity and pathogenesis. Here we report the antiviral properties of ZAP against SRMV, a single-stranded negative-stranded RNA virus of the genus Morbillivirus. ZAP expression was significantly induced in sheep endometrial epithelial cells following SRMV infection. ZAP inhibited SRMV replication in cells after infection, while its overexpression in Vero-SLAM cells significantly increased their resistance to SRMV replication. The ZAP protein co-localized with SRMV RNA in the cytoplasm and ZAPresponsive elements were mapped to the 5 ' untranslated region of SRMV nucleocapsid, phosphoprotein, matrix, and fusion. In summary, ZAP confers resistance to SRMV infection by directly targeting viral RNA and inhibiting viral replication. Our findings further extend the ranges of viral targets of ZAP and help elucidate the mechanism of SRMV replication.

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