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One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus

文献类型：外文期刊

第一作者： Liu, Dafei

作者： Liu, Dafei;Ma, Jianzhang;Liu, Dafei;Guo, Dongchun;Hu, Xiaoliang;Li, Zhijie;Liu, Chunguo;Liu, Fei;Li, Zhigang

作者机构：

关键词： canine distemper virus (CDV); canine kobuvirus (CaKoV); canine parvovirus (CPV); one-step triplex PCR/RT-PCR

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （影响因子：1.267；五年影响因子：1.348 ）

ISSN： 0916-7250

年卷期： 2019 年 81 卷 7 期

页码：

收录情况： SCI

摘要： To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 10(2.1) TCID50 for CDV, 10(1.9) TCID50 for CPV and 10(3) copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs.

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