The nuclear-localized PPR protein OsNPPR1 is important for mitochondrial function and endosperm development in rice

文献类型： 外文期刊

第一作者： Hao, Yuanyuan

作者： Hao, Yuanyuan;Wang, Yunlong;Wu, Mingming;Zhu, Xiaopin;Teng, Xuan;Sun, Yinglun;Zhu, Jianping;Zhang, Yuanyan;Jing, Ruonan;Lei, Jie;Li, Jingfang;Bao, Xiuhao;Wang, Chunming;Wang, Yihua;Wan, Jianmin;Wan, Jianmin

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关键词： Floury endosperm; mitochondrial function; nuclear localization; pentatricopeptide repeat protein; retained intron; rice

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN： 0022-0957

年卷期： 2019 年 70 卷 18 期

页码：

收录情况： SCI

摘要： Pentatricopeptide repeat (PPR) proteins constitute one of the largest protein families in land plants. Recent studies revealed the functions of PPR proteins in organellar RNA metabolism and plant development, but the functions of most PPR proteins, especially PPRs localized in the nucleus, remain largely unknown. Here, we report the isolation and characterization of a rice mutant named floury and growth retardation1 (fgr1). fgr1 showed floury endosperm with loosely arranged starch grains, decreased starch and amylose contents, and retarded seedling growth. Map-based cloning showed that the mutant phenotype was caused by a single nucleotide substitution in the coding region of Os08g0290000. This gene encodes a nuclear-localized PPR protein, which we named OsNPPR1, that affected mitochondrial function. In vitro SELEX and RNA-EMSAs showed that OsNPPR1 was an RNA protein that bound to the CUCAC motif. Moreover, a number of retained intron (RI) events were detected in fgr1. Thus, OsNPPR1 was involved in regulation of mitochondrial development and/or functions that are important for endosperm development. Our results provide novel insights into coordinated interaction between nuclear-localized PPR proteins and mitochondrial function.

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