Effect of ultrasonication on the yield, functional and physicochemical characteristics of collagen-II from chicken sternal cartilage

文献类型： 外文期刊

第一作者： Akram, Ayesha Noreen

作者： Akram, Ayesha Noreen;Zhang, Chunhui

作者机构：

关键词： Ultrasonication; Chicken sternal cartilage; Collagen-II; Functional; Physicochemical; Characterization

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2020 年 307 卷

页码：

收录情况： SCI

摘要： The aim of this study was to extract collagen from chicken sternal cartilage with ultrasound for various times to investigate the effects of ultrasonic waves and, cavitation energy on the yield, functional and physicochemical characteristics of collagen. The highest yield of collagen was 84.14% based on protein weight after ultrasonic treatment for 36 min. Using SDS-PAGE, the extracted protein was identified as collagen-II and its structural integrity was observed using scanning electron microscopy. The triple helical structure remained the same, but some changes in the secondary structure were observed after longer ultrasonication treatments (36 min). The amino acid profile exhibited high thermal stability. The rheological and functional characteristics of collagen were also considerably improved after ultrasonication for the optimal period (24 min). The ultrasonication treatment is an effective process for the extraction of collagen-II from land animal co-products, which will further meet the increasing industrial demand.

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