Selection of reference genes for quantitative real-time PCR analysis of photosynthesis-related genes expression in Lilium regale
文献类型： 外文期刊
第一作者： Du, Wenkai
作者： Du, Wenkai;Yuan, Suxia;Liu, Chun;Du, Wenkai;Hu, Fengrong
作者机构：
关键词： Lilium regale; Reference gene; Quantitative real-time PCR; Photosynthesis
期刊名称：PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS （ 影响因子：2.391； 五年影响因子：2.836 ）
ISSN： 0971-5894
年卷期： 2019 年 25 卷 6 期
页码：
收录情况： SCI
摘要： Photosynthesis is closely related to the growth of plants. A stable reference gene is fundamental for studies of the molecular mechanism of photosynthesis in Lilium regale. Therefore, it is very important to select a suitable reference gene for qRT-PCR analysis on genes of photosynthetic system, chlorophyll biosynthetic pathway and chloroplast development in Lilium regale. Three kinds of tissues, leaves and bulbs (abnormal leaves) of tissue culture plantlets and cotyledons of seedlings of the wild-type and mutant Lilium regale were selected as materials for qRT-PCR. Six housekeeping genes were selected as candidate genes from transcriptome sequencing data of the wild-type and yellow seedling lethal mutant of Lilium regale. Finally, the expression stability of six candidate reference genes was analyzed using geNorm, NormFinder, and BestKeeper software, the comparative increment Ct method, and the RefFinder program. The results showed that LrActin2 was the best reference gene for qRT-PCR analysis of photosynthesis-related genes expression in leaves of tissue culture plantlets and seedlings of Lilium regale. This study provided useful data for further research on molecular mechanism of photosynthesis in the Lilium.
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