Development and application of a fiber2 protein-based indirect ELISA for detection of duck adenovirus 3

文献类型： 外文期刊

第一作者： Chen, Cuiteng

作者： Chen, Cuiteng;Wan, Chunhe;Shi, Shaohua;Cheng, Longfei;Chen, Zhen;Fu, Guanghua;Liu, Rongchang;Zhu, Chunhua;Huang, Yu

作者机构：

关键词： Duck adenovirus 3; Fiber2 protein; Prokaryotic expression; Indirect ELISA

期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）

ISSN： 0890-8508

年卷期： 2019 年 48 卷

页码：

收录情况： SCI

摘要： Duck adenovirus 3 (DAdV-3) is a newly identified duck adenovirus that has recently emerged in China. The incidence of duck infection caused by this virus is very high, with very large economic losses to the poultry industry. Thus, there is an urgent need for a serological assay for the specific detection of DAdV-3. To this end, prokaryotic expression of the fiber2 protein of DAdV-3 was used as a coating antigen to establish an indirect enzyme linked immunosorbent assay (ELISA) method for the specific detection of antibodies against DAdV-3. The method was found to be specific, repeatable and more sensitive than the agarose gel precipitation test (AGP). This indirect ELISA method based on the recombinant fiber2 protein may be used for the clinical detection of DAdV-3 infection and for monitoring antibody levels after vaccine immunization and is of great significance for the effective prevention and control of the disease.

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