AidB, a Novel Thermostable N-Acylhomoserine Lactonase from the Bacterium Bosea sp.

文献类型： 外文期刊

第一作者： Zhang, Jun-Wei

作者： Zhang, Jun-Wei;Xuan, Chen-Guang;Guo, Song;Yu, Jin-Feng;Asif, Muhammad;Jiang, Wen-Jun;Zhang, Li-Qun;Lu, Can-Hua;Zhou, Zhi-Gang;Luo, Zhao-Qing

作者机构：

关键词： quorum quenching; AHL lactonase; thermostability

期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.792； 五年影响因子：5.26 ）

ISSN： 0099-2240

年卷期： 2019 年 85 卷 24 期

页码：

收录情况： SCI

摘要： Many Gram-negative bacteria employ N-acylhomoserine lactones (AHLs) as quorum-sensing (QS) signal molecules to regulate virulence expression in a density-dependent manner. Quorum quenching (QQ) via enzymatic inactivation of AHLs is a promising strategy to reduce bacterial infections and drug resistance. Herein, a thermostable AHL lactonase (AidB), which could degrade different AHLs, with or without a substitution of carbonyl or hydroxyl at the C-3 position, was identified from the soil bacterium Bosea sp. strain F3-2. Ultrahigh-performance liquid chromatography analysis demonstrated that AidB is an AHL lactonase that hydrolyzes the ester bond of the homoserine lactone (HSL) ring. AidB was thermostable in the range 30 to 80 degrees C and showed maximum activity after preincubation at 60 degrees C for 30 min. The optimum temperature of AidB was 60 degrees C, and the enzyme could be stably stored in double-distilled water (ddH(2)O) at 4 degrees C or room temperature. AidB homologs were found only in Rhizobiales and Rhodospirillales of the Alphaproteobacteria. AidB from Agrobacterium tumefaciens and AidB from Rhizobium multihospitium (with amino acid identities of 50.6% and 52.8% to AidB, respectively) also showed thermostable AHL degradation activity. When introduced into bacteria, plasmid-expressed AidB attenuated pyocyanin production by Pseudomonas aeruginosa PAO1 and the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Z3-3, suggesting that AidB is a potential therapeutic agent by degrading AHLs. IMPORTANCE A quorum-sensing system using AHLs as the signal in many bacterial pathogens is a critical virulence regulator and an attractive target for anti-infective drugs. In this work, we identified a novel AHL lactonase, AidB, from a soil bacterial strain, Bosea sp. F3-2. The expression of aidB reduced the production of AHL signals and QS-dependent virulence factors in Pseudomonas aeruginosa and Pectobacterium carotovorum. The homologs of AidB with AHL-degrading activities were found only in several genera belonging to the Alphaproteobacteria. Remarkably, AidB is a thermostable enzyme that retained its catalytic activity after treatment at 80 degrees C for 30 min and exhibits reliable storage stability at both 4 degrees C and room temperature. These properties might make it more suitable for practical application.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序